서지주요정보
α-아밀레이즈와 고정화된 글루코아밀레이즈를 이용한 전분의 액화 및 당화 = Liquefaction and saccharification of starch using α-amylase and immobilized glucoamylase
서명 / 저자 α-아밀레이즈와 고정화된 글루코아밀레이즈를 이용한 전분의 액화 및 당화 = Liquefaction and saccharification of starch using α-amylase and immobilized glucoamylase / 안대희.
저자명 안대희 ; Ahn, Dae-Hee
발행사항 [대전 : 한국과학기술원, 1990].
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소장정보

등록번호

8000899

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 9012

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

For the economical production of ethanol from starch it is necessary to run the process continuously from the raw material starch to the end product ethanol. One of the difficulties is the production of glucose from starch employing α-amylase for liquefaction and glucoamylase for saccharification which are currently done batchwise. This process is inefficient with respect to enzyme utilization since the enzyme is not recovered and reused. The overall objective of this work is to develop the method of enzyme immobilization for saccharification. Starch saccharification by immobilized glucoamylase was chosen because of its economic significance. The catalytic activity of immobilized glucoamylase in a packed-bed reactor and a continuous stirred tank reactor have been compared. The differential reactor was used for the determination of the kinetic parameters. Rapid production of high concentration of glucose from liquefied starchy material through the continuous liquefaction and saccharification using settling chamber have been stuied. Factors affecting reactor performance, such as enzyme concentration, substrate concentration, and reactor temperature on the glucose concentration in the product stream have been stuied. The optimal temperature and pH of the liquefaction were 90°C and 6.0, respectively. In saccharification, the optimal temperature and pH were 60°C and 4.8, respectively. In the case of saccharification in batch reactor with a naked barley concentration of 15% and glucoamylase concentration of 0.13% (v/w), 85% hydrolysis was obtained after 18h. The immobilized glucoamylase with chitin in a packed bed reactor gave the saccharification yield of 20% with the dextrin concentration of 10%. In continuous liquefaction and saccharification, the glucose yield was 17% for the liquefied starch with naked barley concentration of 5%.

서지기타정보

서지기타정보
청구기호 {MCE 9012
형태사항 ix, 76 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Dae-Hee Ahn
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 72-76
주제 Immobilized enzymes.
발효. --과학기술용어시소러스
고정화 효소. --과학기술용어시소러스
Fermentation.
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