서지주요정보
세파렉신 반합성효소의 활성이 증가된 돌연변이주의분리및 특성 = Improvement of cephalexin semi-synthesis bymutagenesis
서명 / 저자 세파렉신 반합성효소의 활성이 증가된 돌연변이주의분리및 특성 = Improvement of cephalexin semi-synthesis bymutagenesis / 박기용.
저자명 박기용 ; Park, Ki-Yong
발행사항 [대전 : 한국과학기술원, 1990].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

8000821

소장위치/청구기호

학술문화관(문화관) 보존서고

MBE 9009

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Improvement of the Activity of Cephalexin Semi-synthesizing Enzyme was attempted by the NTG mutagenesis. Two systems of Cephalexin acylase were used. In Xanthomonas citri IF03835, The conditions for Mutant screening were adjusted. Two mutants having higher activity of cephalexin semi-synthesis than that of the- wild type were selected. In E. coli SH2 having a gene for cephalexin acylase from Bacillus megaterium ATCC14945 on pUC19 plasmid, two tester organisms, E. coli KL16-21 and S. aureus ATCC6583P, with different sensitivity cephalexin (KL16-21 > S. aureus) were used for soft-agar bioassay. Two mutants SH2B and SH2D were obtained from the first round mutagenesis. And using SH2B as parent strain, two more mutants SH2B1, SH2B7 were selected. In Soft-agar Bioassay and Penicillin G hydrolysis assay, the mutants showed higher activity than wild type. In vitro recombination mapping showed that the mutated sites of SH2B, SH2B1 and SH2B7 were located in the C-terminal region of BCA gene. Using shuttle-vector, the BCA genes of SH2B, SH2D, SH2B1 and SH2B7 were transferred to Bacillus subtilis DB104. Higher activity than that of the wild type was detected in B. subtilis having mutated acylase than wild type. The partially purified enzyme from B. subtilis also showed that the mutated acylase had higher activity than wild type enzyme.

서지기타정보

서지기타정보
청구기호 {MBE 9009
형태사항 [vi], 74 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Ki-Yong Park
지도교수의 한글표기 : 박찬규
지도교수의 영문표기 : Chan-Kyu Park
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 참고문헌 : p. 69-74
주제 Mutation (Biology)
Antibiotics.
Enzymes industry.
세팔렉신. --과학기술용어시소러스
효소적 합성. --과학기술용어시소러스
돌연변이. --과학기술용어시소러스
QR CODE qr code