서지주요정보
고농도 아크릴로니트릴에 내성이 있는 고정화 brevibacterium CH2에 의한 아크릴아마이드의 생산 = Production of acrylamide by immobilized brevibacterium CH2 with high acrylonitrile concentration tolerance
서명 / 저자 고농도 아크릴로니트릴에 내성이 있는 고정화 brevibacterium CH2에 의한 아크릴아마이드의 생산 = Production of acrylamide by immobilized brevibacterium CH2 with high acrylonitrile concentration tolerance / 이처영.
저자명 이처영 ; Lee, Cheo-Young
발행사항 [서울 : 한국과학기술원, 1989].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

4105544

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 8925

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

A bacterial strain having higher nitrile hydratase activity, named as $\underline{Brevibacterium}$ sp. CH2, was developed by repeating the cultivation of $\underline{Brevibacterium}$ sp. CHl in the broth containing 1.75%(v/v) acrylonitrile. The properties of the strain was greatly improved. The specific nitrile hydratase activity of the strain increased up to 36 units/mg of dry cell weight, more than 3.6 times higher than that of $\underline{Brevibacterium}$ sp. CHl and the enzyme was not inhibited by the substrate, acrylonitrile concentration of 6% (v/v). The effects of temperature and pH on the activity of free and immobilized cells were investigated. The substrate specificity and the enzyme reaction kinetics such as the substrate and product inhibition were also studied. The conversion yield was nearly 100% with a trace amount of acrylic acid produced. The strain had high nitrile hydratase activity for acrylonitrile, but amidase activity for acrylamide was negligible. In batch cultivation the maximum specific growth rate of 0.24 $h^{-1}$ was obtained at a glucose concentration of 15 g/L and the final cell density 15.4 g/L. In fed-batch culture the cell density increased up to 80 g of dry cell mass/L by regulatory substrate feeding. Continuous production of acrylamide was carried out using the whole cells of $\underline{Brevibacterium}$ sp. CH2 immobilized with polyacrylamide in a two-stage packed bed reactor. The volumetric productivity of the reactor was 93.2 g/L.h with more than 50% conversion. For maximum acrylamide productivity, acrylonitrile concentration and the reaction temperature were 6%(v/v) and 4℃ respectively.

서지기타정보

서지기타정보
청구기호 {MCE 8925
형태사항 vii, 91 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Cheo-Young Lee
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 85-89
주제 Biotechnology.
고정화 세포. --과학기술용어시소러스
생물학적 생산. --과학기술용어시소러스
Immobilized cells.
QR CODE qr code