서지주요정보
CheY의 아세틸화와 대장균의 주화성 = CheY acetylation and chemotaxis in escherichia coli K-12
서명 / 저자 CheY의 아세틸화와 대장균의 주화성 = CheY acetylation and chemotaxis in escherichia coli K-12 / 명현군.
저자명 명현군 ; Myeong, Hyeon-Koon
발행사항 [대전 : 한국과학기술원, 1992].
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등록번호

8002600

소장위치/청구기호

학술문화관(문화관) 보존서고

MBE 92009

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초록정보

The CheY protein, a signaling protein involved in bacterial chemotaxis, was purified using Affi-gel blue column and Sephacryl S-200 gel filtration column. Purified CheY proteins were acetylated in vitro by acetyl-CoA synthetase(ACS; isolated from yeast), and coenzyme A inhibited the acetylation of CheY. Urea gel system can separate modified form unmodified one by charge differences between protein species. When the acetylated CheY was subjected to urea polyacylamide gel lectrophoresis(urea-PAGE), two types of acetylated form detected. This result indicates that the CheY protein have two sites for acetylation, and that the sites are basic amino acid(likely to be Lys.). Cibacron blue dye-binding proteins were frationated from E. coli mutant strain lacking all the chemotaxis components that were induced by acetate. This fraction could acetylate CheY in the absence of ACS(isolated from yeast). This result implies that the acetylation of CheY can take place in E. coli. The CheY protein is phosphorylated by CheA as a part of the chemotactic signal transtuction pathway of E.coli. Phosphorylation of CheY has been proposed to occur in Asp57. When phosphorylated CheY were subjected to urea-PAGE, three types of modified forms were detected. This result suggests that CheY has more than one site for phosphorylation.

서지기타정보

서지기타정보
청구기호 {MBE 92009
형태사항 v, 43 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hyeon-Koon Myeong
지도교수의 한글표기 : 유욱준
지도교수의 영문표기 : Ook-Joon Yoo
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 참고문헌 : p. 41-43
주제 Chemotaxis.
Cellular signal transduction.
Acetylation.
아세틸화. --과학기술용어시소러스
주화성. --과학기술용어시소러스
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