서지주요정보
반사 간섭 분광해석을 이용한 미량단백질 검출 방법에 관한 연구 = Micro detection of protein using spectral analysis of reflection interference
서명 / 저자 반사 간섭 분광해석을 이용한 미량단백질 검출 방법에 관한 연구 = Micro detection of protein using spectral analysis of reflection interference / 이광천.
저자명 이광천 ; Lee, Kwang-Chun
발행사항 [대전 : 한국과학기술원, 2003].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8014579

소장위치/청구기호

학술문화관(문화관) 보존서고

MME 03082

휴대폰 전송

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이용가능

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반납예정일

초록정보

Now days, study on protein analysis has accomplished to understand for biomolecular interaction. But, when protein is analyzed, protein is limited to amplify. Thus micro detection system of protein is demanded in protein analysis in biomolecular. This paper introduce to micro detection of protein using spectral analysis of reflection interference. An optical biosensor using reflection interference is a non-destructive, label-free, and versatile technique in liquid and gaseous phase, that is well suited for monitoring biomolecular interactions. To increase sensitivity, this paper is different from previous method for reflection interference measurement using front side incidence. And this paper uses an antigen-antibody interaction to measure sensitively the specific protein. Biological material deposited at the surface during binding event increases the optical thickness of the interference layer. Increment of the optical thickness related to interference pattern change. In this paper, the optical thickness is calculated through spectral analysis using model fitting. The results show that thickness for antibody is about 12nm and thickness for antibody and antigen is about 19nm. Finally, the micro detection of protein is detected by the calculated optical thickness.

서지기타정보

서지기타정보
청구기호 {MME 03082
형태사항 v, 51 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Kwang-Chun Lee
지도교수의 한글표기 : 김수현
지도교수의 영문표기 : Soo-Hyun Kim
학위논문 학위논문(석사) - 한국과학기술원 : 기계공학전공,
서지주기 참고문헌 : p. 50-51
주제 반사간섭
분광해석
단백질
항원-항체 반응
reflection interference
spectral analysis
protein
antigen-antibody reaction
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