서지주요정보
Monolayer and suspension cultures of HEK 293 cells for recombinant adenovirus production for gene therapy : effect of“cell passage number”= 유전자 치료용 재조합 아데노바이러스의 생산에 미치는 293 부착 세포와 293 부유 세포의 passage에 따른 영향
서명 / 저자 Monolayer and suspension cultures of HEK 293 cells for recombinant adenovirus production for gene therapy : effect of“cell passage number”= 유전자 치료용 재조합 아데노바이러스의 생산에 미치는 293 부착 세포와 293 부유 세포의 passage에 따른 영향 / Myung-Seop Lee.
저자명 Lee, Myung-Seop ; 이명섭
발행사항 [대전 : 한국과학기술원, 2001].
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등록번호

8011594

소장위치/청구기호

학술문화관(문화관) 보존서고

MBS 01008

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초록정보

In recent years, the need for the production of recombinant adenovirus for gene therapy is constantly increasing. To produce E1-deficient recombinant adenovirus in high titer, HEK (Human Embryo Kidney) 293 cells which support the propagation of E1-deficient adenovirus are required. Because the 293 cells transfected with the left 11% of the adenoviral genome express E1A and E1B viral gene products, they are permissive for growth of E1-deficient adenovirus. The 293 cells are obtained at the passage number of 31 from ATCC, and the cell of successive passages are required to produce adenovirus in large scale. Thus, we investigated the effect of passage number of 293 cells on cell growth and adenovirus production. In monolayer culture, the cell size of 293 cells became smaller and the specific growth rate (μ) became faster as the passage number increased. The μ at the passage number of 73 was increased approximately 2.5-fold, compared with that at the passage number of 43. In monolayer culture, the adenovirus production was also influenced by cell passage number. The virus titer obtained from 293 cells at the passage number of 66 was increased approximately 10-fold, compared with that at the passage number of 44. Furthermore, the optimal time for virus harvest post-infection became shorter for the cells at the later passage. In suspension culture, the cell size and μ of 293 cells did not change significantly as passage number increased up to 35. Taken together, the passage number of 293 cells influenced cell growth and adenovirus production in the monolayer culture. Furthermore, replication-competent adenovirus was not detected during cell passages tested in monolayer culture.

서지기타정보

서지기타정보
청구기호 {MBS 01008
형태사항 vi, 58 p. : 삽도 ; 26 cm
언어 영어
일반주기 저자명의 한글표기 : 이명섭
지도교수의 영문표기 : Gyun-Min Lee
지도교수의 한글표기 : 이균민
학위논문 학위논문(석사) - 한국과학기술원 : 생물과학과,
서지주기 Reference : p. 50-56
주제 Adenovirus
아데노바이러스
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