서지주요정보
고정화한 Phanerochaete chrysosporium을 이용한 다환방향족 화합물의 분해에 관한 연구 = Biodegradation of PAHs (polycyclic aromatic hydrocarbons) using immobilized phanerochaete chrysosporium
서명 / 저자 고정화한 Phanerochaete chrysosporium을 이용한 다환방향족 화합물의 분해에 관한 연구 = Biodegradation of PAHs (polycyclic aromatic hydrocarbons) using immobilized phanerochaete chrysosporium / 서윤수.
저자명 서윤수 ; Seo, Yoon-Soo
발행사항 [대전 : 한국과학기술원, 2000].
Online Access 비공개원문

소장정보

등록번호

8011136

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 00016

휴대폰 전송

도서상태

이용가능

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반납예정일

초록정보

This study was aimed to enhance the PAH biodegradation rate by repeated-batch treatment using immobilized Phanerochaete chrysosporium. In batch operations, the degradation rates of anthracene and pyrene in their mixture were 1.74 and 1.67 mg/L·day, respectively. Repeated-batch operations were composed of two steps. The first step was to increase the concentration of immobilized cells using the growth medium, and the second step was to degrade the PAHs in another type of medium for PAH degradation. For cell immobilization, celite bead (100~500 ㎛) was used as the support material. To obtain high cell concentrations, the composition of the growth medium and growth conditions were optimized. As a result, 28.0∼30.0 g/L of cell concentration was obtained in the first step, which corresponded to 109∼118 mg-cell/g-bead of cell loading. In the repeated-batch operations with 30 mg/L of pyrene, the maximum degradation rate was 6.58 mg/L·day. As the number of batch increased, the cell concentration of immobilized cells significantly decreased, and the degradation rate and specific activity gradually increased to a maximum value and then decreased. To revive PAH degrading activity and cell mass, cultivation using the growth medium instead of the PAH-degrading medium was carried-out just for one batch. This led to a significantly recovered cell mass and specific activity although not to the original levels. In the repeated-batch operations for degrading anthracene, a similar trend was observed. The maximum degradation rate was 5.36 mg/L·day. In the repeated-batch operations for degrading anthracene and pyrene simultaneously, the degradation rates for both of them were lower than those when they were treated separately. The maximum degradation rates of pyrene and anthracene were 4.29 and 4.46 mg/L·day, respectively. These values were about 2.5 times higher than those in the batch treatment above.

서지기타정보

서지기타정보
청구기호 {MCHE 00016
형태사항 vii, 60 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Yoon-Soo Seo
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 56-60
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