서지주요정보
Effects of different mRNA 3'-untranslated regions on hammerhead ribozyme efficiency in vivo = 전령 RNA의 3'-비번역 염기열에 따른 생체내 망치머리형 리보핵산효소 효율의 변화
서명 / 저자 Effects of different mRNA 3'-untranslated regions on hammerhead ribozyme efficiency in vivo = 전령 RNA의 3'-비번역 염기열에 따른 생체내 망치머리형 리보핵산효소 효율의 변화 / Young-Hee Sohn.
저자명 Sohn, Young-Hee ; 손영희
발행사항 [대전 : 한국과학기술원, 1999].
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소장정보

등록번호

8009961

소장위치/청구기호

학술문화관(문화관) 보존서고

MBS 99012

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초록정보

880-01The efficiency of ribozyme function depends on the promoter as well as the stability and localization of the transcript. Thus expression cassette determine the functional activity of ribozymes by controlling their intracellular colocalization. We have studied hammerhead ribozyme function suppressing Hepatitis B Virus X gene. Especially, we have designed the expression cassette including β- and γ-actin 3-UTR(untranslated region). Actin 3-UTR sequences are responsible for localization of mRNA and we acquired these sequences by PCR with HeLa cDNA library. pRc/RSV was used as mammalian cell expression vector and several plasmids containing riobzyme and target flanked by each 3-UTR were constructed. After each kind of ribozyme and target were paired off, these vectors were transiently transfected into HeLa cell and ribozyme activities were detected by β-galactosidase assay. When the cells were transfected with vectors including 3-UTR in either target or ribozyme, the activity of ribozyme was reduced. But the cells were tranfected with vectors including the same kind of 3-UTR in both target and ribozyme, the activity of ribozyme was slightly enhanced.

서지기타정보

서지기타정보
청구기호 {MBS 99012
형태사항 [v], 48 p. : 삽도 ; 26 cm
언어 영어
일반주기 저자명의 한글표기 : 손영희
지도교수의 영문표기 : Jong-Kyeong Chung
공동교수의 영문표기 : Chang-Won Kang
지도교수의 한글표기 : 정종경
공동교수의 한글표기 : 강창원
학위논문 학위논문(석사) - 한국과학기술원 : 생물과학과,
서지주기 Reference : p. 44-46
주제 Hammerhead ribozyme
Actin
3'-untranslated region
Localization
망치머리형 리보핵산 효소
액틴
3'-비번역 부위
배치
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