서지주요정보
Protein kinase activity of Kaposi's sarcoma associated herpesvirus (KSHV) ORF 36 = 카포시 육종 허피스 바이러스의 인산화 효소에 관한 연구
서명 / 저자 Protein kinase activity of Kaposi's sarcoma associated herpesvirus (KSHV) ORF 36 = 카포시 육종 허피스 바이러스의 인산화 효소에 관한 연구 / Jun-Soo Park.
저자명 Park, Jun-Soo ; 박준수
발행사항 [대전 : 한국과학기술원, 1999].
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소장정보

등록번호

8009960

소장위치/청구기호

학술문화관(문화관) 보존서고

MBS 99011

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초록정보

The Kaposi's Sarcoma associated Herpesvirus(KSHV/HHV-8) is a γ herpesvirus that is implicated in the pathogenesis of Kaposi's sarcoma. The nucleotide sequence of the KSHV open reading frame 36 predicts a polypeptide with significant sequence homology to the catalytic domain of known protein kinases. To determine whether the KSHV ORF36 polypeptide is a protein kinase, we have expressed it in COS-1 cell as a fusion with glutathione S-transferase. Affinity precipitated preparations of fusion protein were found to be autophosphorylated. Mutation of lysine 108 to glutamine within the conserved kinase catalytic domain II decreased protein kinase activity dramatically, supporting the notion that the protein kinase activity is inherent to the ORF36 polypeptide. The divalent cation preference of KSHV ORF36 was determined by measuring kinase activity in the presence of various concentrations of $Mn^{2+} or Mg^{2+}$, and KSHV ORF36 was shown to prefer $Mn^{2+} to Mg^{2+} for maximal kinase activity. Phosphoamino acid analysis showed that KSHV ORF36 fusion protein was phosphorylated on a serine residue, implicating that KSHV ORF36 encoded a serine/threonine kinase. To define the subcellular localization of KSHV ORF36, we expressed Green Fluorescence Protein(GFP) KSHV ORF36 fusion protein in COS-1 cell, and subsequent microscopy experiment showed that KSHV ORF36 localized in the nucleus.

서지기타정보

서지기타정보
청구기호 {MBS 99011
형태사항 v, 45 p. : 삽도 ; 26 cm
언어 영어
일반주기 저자명의 한글표기 : 박준수
지도교수의 영문표기 : Joon-Ho Choe
지도교수의 한글표기 : 최준호
학위논문 학위논문(석사) - 한국과학기술원 : 생물과학과,
서지주기 Reference : p. 36-41
주제 KSHV
Protein kinase
Herpesvirus
Phosphorylation
카포시 육종
허피스 바이러스
인산화 효소
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