서지주요정보
갈락토오즈 배지에서 재조합 효모에 의한 inulinase 생산 모델링 = Modelling of inulinase production by a recombinant yeast in galactose medium
서명 / 저자 갈락토오즈 배지에서 재조합 효모에 의한 inulinase 생산 모델링 = Modelling of inulinase production by a recombinant yeast in galactose medium / 나정걸.
저자명 나정걸 ; Na, Jeong-Geol
발행사항 [대전 : 한국과학기술원, 1996].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8006565

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 96010

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

An unstructured model has been developed for the batch culture of a recombinant yeast with a GAL promoter, in galactose media containing ethanol. The major goal was modelling of the catabolic repression of galactose on ethanol consumption, inhibition on cell growth by ethanol, and plasmid instability. Batch cultures were carried out for several initial concentrations of galactose and ethanol for this purpose. The decrease in the plasmid-bearing cell population during the culture was observed to be severe with a medium containing galactose as carbon source without ethanol(galactose-only medium). Its fraction at the end of the run(at 38 hours) was no more than 32%. The major cause of such reactor instability was found to be the plasmid instability since the depression of plasmid-bearing cell growth, which is another potential cause for the reactor instability, was observed to be negligible. The plasmid loss frequency could be represented appropriately by a saturation type function of specific galactose consumption rate. When galactose and ethanol existed concurrently in the fermentor, ethanol consumption rate was repressed by galactose when its concentration was high. As galactose was consumed and its concentration became below certain level, galactose and ethanol started to be consumed simultaneously. When ethanol concentration was above 40 g/L, galactose consumption and thus cell growth stopped. To observe the roles of ethanol, batch culture with ethanol-only media was carried out. Ethanol was an inhibitor on cell growth as well as the carbon source. The specific growth rate with ethanol was lower than galactose, but cellular yield for ethanol was higher. The plasmid-bearing cell and the plasmid-free cell differed from each other in terms of ethanol consumption kinetics. But, the kinetics of catabolic repression by galactose on ethanol consumption was almost the same for both cell types. A model including all the aspects mentioned above was proposed. Some model parameters were determined directly from the experimental data. The other parameters were determined initially by an eye-bowling approach and eventually by using an evolutionary strategy which was known to be easy to apply and robust. Although our model was quite complex and had many parameters, the result of parameter determination was satisfactory and the model with those estimated parameters fit the experimental data quite well.

서지기타정보

서지기타정보
청구기호 {MCHE 96010
형태사항 ix, 60 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Jeong-Geol Na
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 58-60
주제 재조합 효모 모델링
evolutionary 알고리듬
Modelling of recombinant yeast
Evolutionary algorithm
QR CODE qr code