서지주요정보
Loss of productivity of transfectoma producing chimeric antibody during a long-term culture = 트랜스펙토마의 장기 배양시 항체 생산력 감소에 관한 연구
서명 / 저자 Loss of productivity of transfectoma producing chimeric antibody during a long-term culture = 트랜스펙토마의 장기 배양시 항체 생산력 감소에 관한 연구 / Jae-Hoon Kim.
저자명 Kim, Jae-Hoon ; 김재훈
발행사항 [대전 : 한국과학기술원, 1996].
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소장정보

등록번호

8006702

소장위치/청구기호

학술문화관(문화관) 보존서고

MBS 96006

휴대폰 전송

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초록정보

The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity ($q_{Ab}$) of transfectoma decreased by 60-88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the $q_{Ab}$ of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the $q_{Ab}$ of P at the beginning of long-term culture, the $q_{Ab}$ of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased $q_{Ab}$ of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased $q_{Ab}$ of P during the long-term culture.

서지기타정보

서지기타정보
청구기호 {MBS 96006
형태사항 vi, 42 p. : 삽도 ; 26 cm
언어 영어
일반주기 저자명의 한글표기 : 김재훈
지도교수의 영문표기 : Gyun-Min Lee
지도교수의 한글표기 : 이균민
학위논문 학위논문(석사) - 한국과학기술원 : 생물과학과,
서지주기 Reference : p. 34-37
주제 Chimeric antibody
Stability
Long-term culture
RT-PCR
Southern Blot
재조합 항체
안정성
장기배양
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