서지주요정보
박테리오파지의 용해작용을 이용한 재조합 대장균으로부터의 PHB 회수에 관한 연구 = The recovery of PHB from recombinant escherichiacoli using lysis function of bacteriophage
서명 / 저자 박테리오파지의 용해작용을 이용한 재조합 대장균으로부터의 PHB 회수에 관한 연구 = The recovery of PHB from recombinant escherichiacoli using lysis function of bacteriophage / 정옥희.
저자명 정옥희 ; Jeung, Ok-Hee
발행사항 [대전 : 한국과학기술원, 1995].
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소장정보

등록번호

8005815

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 95032

SMS전송

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초록정보

An autolytic system based on a thermally inducible phage lambda, λHL1, has been applied for the recovery of poly(3-hydroxybutyric acid), PHB from a recombinant E. coli, XL1-Blue. The lytic capability of λHL1 was evaluated in flask culture for a lysogen, XL1-Blue(λHL1) which had been constructed by infecting XL1-Blue with λHL1. When the optical density at 600 nm ($OD_{600}$) of the culture broth reached 0.2, cell lysis was induced by increasing the culture temperature from 30℃ to 42℃. We observed that the $OD_{600}$ decreased to a negligible level in an hour after the thermal induction, as most of the cells were destroyed by the autolytic system. A series of flask cultures were also carried out with XL1-Blue(λHL1, pSYL105) which had been constructed by transforming XL1-Blue(λHL1) with pSYL105, a plasmid containing PHB synthesis gene. We observed that the lytic efficiency was about 80% when thermal induction was done at an $OD_{600}$ of 0.2, and that the existence of the plasmid in the cells inhibited to some extent, the lytic capability of λHL1 even with no significant level of PHB accumulation. When we tried thermal induction at an $OD_{600}$ of 0.34 - 0.4, only a low level of lytic efficiency of less than 60% was observed. We tried chemical induction in addition to thermal induction to obtain a much better lytic efficiency. In another series of experiments with XL1-Blue(λHL1,pSYL105), we investigated the interaction between the expression of λHL1 and the accumulation of PHB in the cells. The lytic efficiency decreased significantly from 60% to 30% as the induction was delayed from at 6 hours to at 12 hours to allow more PHB to accumulate in the cells. Also, the existence of λHL1 in the cells was found to inhibit to some extent, PHB accumulation.

서지기타정보

서지기타정보
청구기호 {MCHE 95032
형태사항 ix, 52 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Ok-Hee Jeung
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 50-52
주제 Escherichia coli.
Bacteriophages.
대장균. --과학기술용어시소러스
박테리오파지. --과학기술용어시소러스
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