서지주요정보
Isolated methylotroph로부터 PHB 및 P(3HB-co-3HV)의 생산 = Production of PHB and P(3HB-co-3HV) by isolated methylotroph
서명 / 저자 Isolated methylotroph로부터 PHB 및 P(3HB-co-3HV)의 생산 = Production of PHB and P(3HB-co-3HV) by isolated methylotroph / 김필.
저자명 김필 ; Kim, Pil
발행사항 [대전 : 한국과학기술원, 1994].
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소장정보

등록번호

8004396

소장위치/청구기호

학술문화관(문화관) 보존서고

MBT 94005

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초록정보

Bacteria using methanol were isolated from methanol contaminated soils in Gumi plant complex. The most promising culture was selected based on rapid growth and capacity of PHB accumulation. It was tested that various kinds of precursors such as acids, alcohols, esters, and alkanes could be incorporated in PHB, and certified by gas chromatograph. Only valeric acid could be incorporated in PHB. Selected one was cultivated in various kinds of nutrient limited condition and excess carbon source, because PHB is carbon reserve material. These condition could accelerate accumulation of PHB, and especially nitrogen limitation cause highest product yield(P/X = 0.55). For producing PHB in optimal condition, optimal carbon concentration and pH were selected by Bioscreen C. Optimal methanol concentration was 0.1 to 1.55% (v/v), Ks and Ki in Andrews equation were 0.004, 1.55% respectively. Optimal pH range was 7.0 to 8.0. From above data, fed-batch culture was performed in optimal condition by using D.O.-stat and pH-stat method. Valeric acid was added to 0.2% (v/v) at the begining of stationary phase. Final cell concentration was 45 g/l, P(3HB-co-3HV) was 20.3 g/l, and 3HV fraction in P(3HB-co-3HV) was 4.5%.

서지기타정보

서지기타정보
청구기호 {MBT 94005
형태사항 v, 51 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Pil Kim
지도교수의 한글표기 : 이균민
지도교수의 영문표기 : Gyun-Min Lee
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 참고문헌 : p. 48-50
주제 Methanol.
Cell culture.
Methylotrophic microorganisms.
메탄올. --과학기술용어시소러스
세포 배양. --과학기술용어시소러스
메틸로트로프. --과학기술용어시소러스
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