서지주요정보
효소를 이용한 유기용매에서의 아스파탐 전구체의 합성 = Enzymatic synthesis of aspartame precursor in organic media
서명 / 저자 효소를 이용한 유기용매에서의 아스파탐 전구체의 합성 = Enzymatic synthesis of aspartame precursor in organic media / 박오진.
저자명 박오진 ; Park, Oh-Jin
발행사항 [대전 : 한국과학기술원, 1993].
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소장정보

등록번호

8003882

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 93030

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

A synthetic reaction of dipeptide was carried out in organic solvents, using a thermostable neutral protease. The synthesis of benzyloxycarbonyl-L-aspartic-L-phenylalnine methyl ester, an aspartame precursor, is a typical protease-catalyzed reaction of peptide synthesis. Hydrolytic activity was reversed in nonaueous environment favoring synthesis of peptides, reversal of hydrolysis of peptide bond. Thermodynamic equilibria can be shifted toward condensation by introducing water-miscible and water-immiscible solvents. Synthetic yield and initial reaction rate can be influenced by toxicity and hydrophobicity of solvents. Various organic solvents were tested as reaction media. Thermolysin-catalyzed reaction was implemented in monophasic and biphasic system. There exists a optimal water content that gives the maximum yield. In water-immiscible solvents, distribution of reactants and products between organic and aqueous phase was shown to have effects on synthetic yield. Solvents may inhibit enzymatic activity and immobilization can be used to reduce solvent toxicity and stabilize enzyme and make it possible to use enzyme repeatedly in continuous operation.

서지기타정보

서지기타정보
청구기호 {MCHE 93030
형태사항 vi, 70 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Oh-Jin Park
지도교수의 한글표기 : 양지원
지도교수의 영문표기 : Ji-Won Yang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 65-68
주제 Enzymes.
Aspartame.
효소. --과학기술용어시소러스
아스파라탐. --과학기술용어시소러스
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