서지주요정보
분무탑에서 역미셀을 이용한 세포외와 세포내 단백질의 분리 = Separation of extracellular and intracellular proteins using reverse micelles in spray column
서명 / 저자 분무탑에서 역미셀을 이용한 세포외와 세포내 단백질의 분리 = Separation of extracellular and intracellular proteins using reverse micelles in spray column / 한동훈.
저자명 한동훈 ; Han, Dong-Hoon
발행사항 [대전 : 한국과학기술원, 1993].
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소장정보

등록번호

8003875

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 93023

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

The separation of proteins using Reverse Micelles in spray column was investigated. In this experiment, Extracellular Lipase and Intracellular Uricase were separated from aqueous solution. During the operation, the temperature was maintained at 27℃. For the deternimation of protein concentration, BCA micro assay was used. The amount of water in organic phase was assayed by Karl-Fisher Titrimeter. We determimed the basis factors affecting the separation for extracellular and intracellular proteins such as pH of the aqueous phase, concentration of salt, surfactant and reducing agent mixing chamber. The effect of pH was almost the same as the result of others for Lipase and Uricase. The optimum concentraton of salt for the separation was determined. As the concentration of surfactant increases, two maxima were shown in intracellular type. The amount of proteins in organic phase for intracellular type was increased with an increase in reducing agent. Also the separation in spray column was performed at determined conditions. We investigated the optimum operation conditions, especially good extraction capacity, energy reduction and easy phase separation. The flow rate was optimum near 0.1 ml/sec. According to the increase of the circulation, the amount of extraction was almost constnat. The increase of length in column had positive effect on the amount of extraction. The amount of proteins in orgainc phase was increased with an increment of number of hole in distributor.

서지기타정보

서지기타정보
청구기호 {MCHE 93023
형태사항 [xi], 80 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Dong-Hoon Han
지도교수의 한글표기 : 홍원희
지도교수의 영문표기 : Won-Hi Hong
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 75-80
주제 Separation (Technology)
Reversed micelles.
분리. --과학기술용어시소러스
역미셀. --과학기술용어시소러스
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