서지주요정보
아크릴아마이드의 생물학적 생산을 위한 brevibacterium sp. CH2 균주의 배지 최적화 및 nitrile hydratase 효소의 안정화 = Medium optimization of brevibacterium sp. CH2 and stabilizaition of the nitrile hydratase for biological production of acrylamide
서명 / 저자 아크릴아마이드의 생물학적 생산을 위한 brevibacterium sp. CH2 균주의 배지 최적화 및 nitrile hydratase 효소의 안정화 = Medium optimization of brevibacterium sp. CH2 and stabilizaition of the nitrile hydratase for biological production of acrylamide / 최상교.
저자명 최상교 ; Choi, Sang-Kyo
발행사항 [대전 : 한국과학기술원, 1991].
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소장정보

등록번호

8002255

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 9125

휴대폰 전송

도서상태

이용가능

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반납예정일

초록정보

Optimum culture conditions of Brevibacterium sp. CH2 and stabilizing effect of various organic acids on nitrile hydratase were investigated. The best carbon and nitrogen sources of Brevibacterium sp. CH2 were glucose and a mixture of bacto peptone, yeast extract and malt extract, respectively. The addition of ferric and ferrous ions greatly enhanced nitrile hydratase formation. The effects of nitriles, amides, and acids as an inducer on the formation of nitrile hydratase were investigated. Isobutyramide was the best inducer among those compounds tested. The maximum total activity was 630 units/ml of culture broth and the specific activity was 70 units/mg of dry cells, when Brevibacterium sp. CH2 was cultivated for 21 hours at 30℃ in the following optimized medium; 15g of glucose, 5g of bacto peptone, 3g of yeast extract, 3g of malt extract, 1g of $KH_2PO_4$, 1g of $K_2HPO_4$, 1g of NaCl, 0.5g of isobutyramide, 0.2g of $MgSO_4·7H_2O$, and 0.02g of $FeSO_4·7H_2O$ per liter of distilled water with pH controlled at 7.1. As a result of the medium optimization, the specific activity of nitrile hydratase was 2.2 times increased and the substrate inhibition effect of nitril hydratase was considerably reduced. The new values of kinetic constants are as follows; $V_{max}$:6.31mol/h/g, $K_m$:0.015mol/L, $K_s$:0.55mol/L and $K_p$:0.065 mol/L. The best stabilizing agent among the organic acids tested was n-butyric acid. When 20 mM of n-butyric acid was added in the buffer solution containing suspended cells, the initial enzyme activity was perfectly maintained for 20 days at 5℃. Consequently, we could obtain 400g of acrylamide per 1g of dry cell. However, the strain Brevibacterium sp. CH2 is susceptible to a serious product inhibition. Therefore there exists a room for the improvement of Brevibacterium sp. CH2 by the study of product adaptation method or mutagenetic research, etc.

서지기타정보

서지기타정보
청구기호 {MCE 9125
형태사항 x, 88 p. : 삽도 ; 26 cm
언어 한국어
일반주기 부록 수록
저자명의 영문표기 : Sang-Kyo Choi
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 73-78
주제 Biotechnology.
아크릴아미드. --과학기술용어시소러스
생물학적 생산. --과학기술용어시소러스
Acrylamide.
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