서지주요정보
6-APA의 효소석 생산에 있어서 이상계의 이용 = Utilization of two-liquid phase system in the enzymatic production of 6-APA
서명 / 저자 6-APA의 효소석 생산에 있어서 이상계의 이용 = Utilization of two-liquid phase system in the enzymatic production of 6-APA / 김장규.
저자명 김장규 ; Kim, Jang-Gyu
발행사항 [대전 : 한국과학기술원, 1991].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

8002232

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 9104

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Extractive bioconversions of penicillin G by penicillin acylase were carried out using water-organic solvent two-phase systems. Rekker's hydrophobic fragmental constant method and linear free energy relationship (LFER) were used in estimating partition coefficients of the substrate and the product in some two-phase systems. In this study organic solvents which have high partition coefficient for product (phenylacetic acid) which inhibits enzyme reaction were taken into account first and then, considering the general rule that solvents whose log P values are more than 2 are favored for enzyme activity, n-octanol (log P, 2.9) and cyclohexanol (log P, 1.5) were chosen for the experiment. Cyclohexanol turned out to be useless due to its serious deactivation effect on enzyme. Octanol worked well especially when pH was not controlled. Thermodynamic theory could successfully predict the 6-APA yield when the reaction was completed so fast that β-lactam ring degradation was negligible.

서지기타정보

서지기타정보
청구기호 {MCE 9104
형태사항 ix, 72 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Jang-Gyu Kim
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 70-72
주제 Enzymes.
페니실린. --과학기술용어시소러스
효소. --과학기술용어시소러스
Penicillin.
QR CODE qr code