서지주요정보
Ganglioside Gm1을 함유한 불포화 PE immunoliposome 의 제조와 특성 = Preparation and characteristics of unsaturated PE immunoliposome incorporated with ganglioside Gm1
서명 / 저자 Ganglioside Gm1을 함유한 불포화 PE immunoliposome 의 제조와 특성 = Preparation and characteristics of unsaturated PE immunoliposome incorporated with ganglioside Gm1 / 김창수.
저자명 김창수 ; Kim, Chang-Soo
발행사항 [대전 : 한국과학기술원, 1991].
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등록번호

8002233

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 9105

SMS전송

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초록정보

The stabilities of immunoliposomes incorporated with variable amounts of ganglioside $G_{M1}$, were investigated as a function of time and temperature, by observing absorbance of vacant liposomes and calcein release from entrapped liposomes. The bilayer of unsaturated PE(dioleoylphosphatidylethanolamine:DOPE), an hexagonal phase forming lipid, which dose not form stable liposomes at physiological temperature and pH, were formed when palmitoyl-immunoglobulin G(IgG) (2.5*$10^{-4}$ mol/DOPE mol) added, but destabilized within several hours. The incorporation of ganglioside $G_{M1}$ into immunolisome, enhanced the stabilities of bilayers, but aggregation and fusion between liposomes may be also promoted without any leaking of calcein. Such rapid aggregation and fusion would be because of the interaction between ganglioside $G_{M1}$ and protein(IgG). The turbidity of immunoliposomes coated with ganglioside $G_{M1}$ increased up to 20mol% $G_{M1}$/DOPE mol, but decreased above the maximum. Such phenomena may be attributed to the disturbance of the bilayer characteristics, i.e. surface charge, layer transition or reorientation of interaction sites, when the concentration of ganglioside $G_{M1}$ increased. The immunoliposomes coated with ganglioside $G_{M1}$ at 5℃ showed the higher stability than samples tested at elevated temperatures. After on week of storage, the precipitants at all temperatures were redispersed by mild sonication, and the redispersed liposomal solutions at lower temperatures maintained the original elution patterns in chromatography but broader distribution at elvated temperatures. Such observations suggested that during storage, the aggregation of liposomes kept at 5℃ is more dominant mechanism, but that the fusion is at elevated temperatures.

서지기타정보

서지기타정보
청구기호 {MCE 9105
형태사항 vii, 63 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Chang-Soo Kim
지도교수의 한글표기 : 김종득
지도교수의 영문표기 : Jong-Duk Kim
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 58-63
주제 Physiology.
리포좀. --과학기술용어시소러스
생리학. --과학기술용어시소러스
Liposomes.
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