서지주요정보
동시고정화 효소를 이용한 sucrose 로부터 gluconate 와 sorbitol 의 생산 = Production of gluconate and sorbitol from sucrose by using immobilized multienzyme complex
서명 / 저자 동시고정화 효소를 이용한 sucrose 로부터 gluconate 와 sorbitol 의 생산 = Production of gluconate and sorbitol from sucrose by using immobilized multienzyme complex / 노현수.
저자명 노현수 ; Ro, Hyeon-Su
발행사항 [대전 : 한국과학기술원, 1991].
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소장정보

등록번호

8001805

소장위치/청구기호

학술문화관(문화관) 보존서고

MBE 9109

휴대폰 전송

도서상태

이용가능

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리뷰정보

초록정보

Gluconate and sorbitol could be produced from glucose and fructose by using glucose-fructose oxidoreductase (GFOR) of the permeabilized cells of Zymomonas mobilis. These substrates, however, appeared to be not suitable for the production of relatively cheap products. Thus, our research was focused on the use of sucrose which was major component of natural sugar sources. Hydrolyzation of sucrose by invertase or other sucrose hydrolyzing enzyme was required for this purpose. Our system has three step reaction; inversion step, oxidoreduction step and gluconate formation step. The use of immobilized multienzyme complex(IMC) has many advantages over single enzyme process. One of these is ability of multi-step reaction. But, estabilishment of operational conditions are very difficult in this process. So, we estabilished several operational conditions; activity ratio, pH, temperature etc. Reactions by IMC were accomplished with different reaction mode. In continuous stirred tank reactor operation, the maximum productivity was reached 4.6g/L·h at dilution rate $0.055h^{-1}$. But, stirred type teactor was disadvantageous for low operational stability. In order to increase the operational stability, recycled packed bed reactor(RPBR) system was adopted that did'nt require stirring. In RPBR, the productivity at recirculation rate=1200ml/h and dilution rate =0.053$h^{-1}$ was 5.2g/L·h. The activity of IMC was maintained totally to 200h of operation time.

서지기타정보

서지기타정보
청구기호 {MBE 9109
형태사항 [vii], 51 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hyeon-Su Ro
지도교수의 한글표기 : 김학성
지도교수의 영문표기 : Hak-Sung Kim
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 참고문헌 : p. 48-51
주제 Bioreactors.
Immuobilized enzymes.
Glucose.
고정화 효소. --과학기술용어시소러스
바이오 리액터. --과학기술용어시소러스
글루코오스. --과학기술용어시소러스
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