서지주요정보
Immunoglobulin G가 함유된 PE-리포솜의 안정성에 미치는 bile salt의 영향 = The effects of bile salts on the stability of PE-liposome incorporated with immunoglobulin G(IgG)
서명 / 저자 Immunoglobulin G가 함유된 PE-리포솜의 안정성에 미치는 bile salt의 영향 = The effects of bile salts on the stability of PE-liposome incorporated with immunoglobulin G(IgG) / 이은옥.
저자명 이은옥 ; Lee, Eun-Ok
발행사항 [대전 : 한국과학기술원, 1990].
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소장정보

등록번호

8000909

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 9017

SMS전송

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초록정보

The effects of Bile salts (BS) on the stability of dioleoylphosphatidylethanolamine (DOPE) lipo somes were investigated, observing light absorbance of vacant liposome and calcein release from entrapped lipo some. Unilamellar liposomes were prepared by using a small quantity of palmitoyl-immunoglobulin G(IgG) ($2.5\times10^{-4}$ mol/lipid mol) to stabilize the bilayer phase of the unsaturated DOPE which by itself does not form stable liposomes. The destabilization of PE immunoliposomes by papain, clearly demonstrates that the IgG is essential for stabilization of PE bilayer. Approximately 4% of the entrapped calcein was released from the PE liposomes after 1 hr from liposome formation. Absorbance of liposomes decreased depending on the BS/lipid ratio because of the solubilization of lipid molecule in bilayer and the formation of mixed micelles. At very low BS concentrations, BS/lipid aggregates are formed in the outer vesicles monolayer, while at increasing BS concentrations, mixed micelles are formed. Cholate and its conjugates as 3$\alpha$, 7$\alpha$, 12$\alpha$-trihydroxy BS induce half-maximal solubilization of immunoliposome at approximately 2.5-, or 5-fold concentration of the 3$\alpha$, 12$\alpha$-dihydroxy BS. Conjugation of BS with glycine or taurine results in a slight enhancement of their capacity to perturb membranes.

서지기타정보

서지기타정보
청구기호 {MCE 9017
형태사항 vii, 75 p. : 삽도 ; 26 cm
언어 한국어
일반주기 부록 수록
저자명의 영문표기 : Eun-Ok Lee
지도교수의 한글표기 : 정인재
지도교수의 영문표기 : In-Jae Chung
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 67-70
주제 Physiology.
Stability.
생리학. --과학기술용어시소러스
안정성. --과학기술용어시소러스
Bile salts.
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