To compare the promoter recognition ability between $\underline{Escherichia}$ $\underline{coli}$ and $\underline{Bacillus}$ $\underline{subtilis}$ as well as to screen a strong promoter of $\underline{B}$. $\underline{subtilis}$, we have constructed a promoter library of $\underline{B}$. $\underline{subtilis}$ in $\underline{E}$. $\underline{coli}$ using a promoter probe vector p602/8. The library was screened for the presence of chloramphenicol resistance. About eighty chloramphenicol resistant clones were confirmed. The recombinant plasmids isolated from chloramphenicol resistant clones contained chromosomal inserts with size of 130-500 base pairs. Relative strengths of chloramphenicol resistance of each clones in $\underline{E}$. $\underline{coli}$ were compared with control promoters of tact and SPOII on the solid media. The level of chloramphenicol resistance ranged from 80 to 700 ug/ml. Transfering p602/8 derivatives containing cloned DNA fragments into $\underline{B}$. $\underline{subtilis}$, promoter donor cell, promoter activity of cloned DNA fragments were analyzed with chloramphenicol resistance, SDS polyacrylamide gel electrophoresis and assay of chloramphenicol acetyltransferase activity. All cloned DNA fragments showed promoter activity in $\underline{B}$. $\underline{subtilis}$ also. $\underline{B}$. $\underline{subtilis}$ cells harvouring the cloned DNA fragments displayed chlorampheni col resistance from 10 to 150ug/ml on solid media and chloramphenicol acetyltransferase activity from 31 miliunits to 5 units. Some of the cloned Bacillus chromosomal DNA fragments such as BJ 2, BJ 27, BJ 29, showed promoter activity similar to SPOII promoter which is known to a strong promoter in $\underline{B}$. $\underline{subtilis}$, and promoted synthesis of chloramphenicol acetyl transferase from 20 to 25% of total cell proteins. These' promoters have a potential to make foreign genes express efficiently in $\underline{B}$. $\underline{subtilis}$.
$\underline{Escherichia}$ $\underline{coli}$ 와 $\underline{Bacillus}$ $\underline{subtilis}$ 내에서 RNA 중합효소와 촉진제 사이의 상호작용을 비교하고, 강력한 $\underline{B}$. $\underline{subtilis}$ 촉진제를 찾아내기위해, 촉진제 검색벡터 p602/8 을 이용하여, $\underline{B}$. $\underline{subtilis}$ 염색체로부터 촉진제들을 $\underline{B}$. $\underline{coli}$ 내로 클로닝했다. 많은 형질 전환주중에 80 개의 클로람페니콜 내성 형질 전환주를 확정했고, $\underline{B}$. $\underline{coli}$ 에서 내성정도는 고체배지 ml 당 80-700 ug이었다. 클로닝된 DNA 절편의 크기는 130-500 bp 였다. 클로닝된 DNA절편을 갖고있는 p602/8 유도체들을 $\underline{B}$. $\underline{subtilis}$ 로 옮겨서, $\underline{B}$. $\underline{subtilis}$ 내에서 이 DNA 절편들의 촉진제 활성을 분석했다. 모든 클로닝된 DNA 절편들은 $\underline{B}$. $\underline{subtilis}$ 에서도 촉진제 활성을 보였다. 클로람페니콜내성 정도는 고체배지 ml당 10-150ug이었고, 클로람페니콜 아세틸 전이효소 활성은 31mU에서 5U였다. 이 DNA 절편들중에서 BJ2, BJ27, BJ29 들은 $\underline{B}$. $\underline{subtilis}$에서 강력한 촉진제라고 알려진 SPO II 촉진제와 비슷한 활성을 보였고, 이들은 총 세포 단백질의 20-27 % 의 클로람페니콜 아세틸 전이효소의 합성을 유도했다. 이 DNA 절편들은 $\underline{B}$. $\underline{subtilis}$ 내에서 외부유전자들을 발현시키는데에 이용할 수 있다.
