서지주요정보
크로마토포커싱에 의한 단백질의 분리와 모사 = Protein separation by chromatofocusing and computer simulation
서명 / 저자 크로마토포커싱에 의한 단백질의 분리와 모사 = Protein separation by chromatofocusing and computer simulation / 천석원.
저자명 천석원 ; Chon, Suk-Won
발행사항 [서울 : 한국과학기술원, 1989].
Online Access 원문보기 원문인쇄

소장정보

등록번호

4105551

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 8932

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

The separation process of proteins in a column packed with an ion exchanger PBE94 under the internal pH gradient was simulated experimentally by the elution of model proteins and by the computer calculation. Under the elution of eluent polybuffer, the linear pH gradient was developed and the elution pH's of myoglobin I and II were observed near the PI's of each proteins. For myoglobin I, the protein fed at different time, was focused into single peak at elution, and the elution times of model proteins appeared near their isoelectric points. For the smooth and linear pH gradients of the systems having interaction with ion exchangers and mixed buffers, a differential model with the regulation by buffers were proposed incorperating a mixed model buffers. The calculated linear pH gradients showed good agreements with experimental results and sensitive to dimensionless groups and Ke. In computer simulation of the protein separation and focusing, the coupled differential model with the pH gradient and the protein elution were also proposed. According to our calculation, the protein elution and separations were not only influenced by the Peclet number but also dimensionless number PO but the internal patterns of protein elutions indicated the strong interaction of protein and gel.

서지기타정보

서지기타정보
청구기호 {MCE 8932
형태사항 x, 108 p. : 삽도 ; 26 cm
언어 한국어
일반주기 Appendix : A, The properties of proteins. - B, Bi-tridiagonal algorithm. - C, Source program for chromatofocusing
저자명의 영문표기 : Suk-Won Chon
지도교수의 한글표기 : 김종득
지도교수의 영문표기 : Jong-Duk Kim
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 81-84
주제 Separation (Technology)
모델링. --과학기술용어시소러스
분리 (조작) --과학기술용어시소러스
Computer simulation.
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