서지주요정보
Glucose isomerase activity 를 가진 arthrobacter sp.를 고정화시킨 실관 효소 반응기를 통한 과당의 생산 = Production of fructose using hollow fiber enzyme reactor with immobilized arthrobacter sp. possessing glucose isomerase activity
서명 / 저자 Glucose isomerase activity 를 가진 arthrobacter sp.를 고정화시킨 실관 효소 반응기를 통한 과당의 생산 = Production of fructose using hollow fiber enzyme reactor with immobilized arthrobacter sp. possessing glucose isomerase activity / 오영호.
저자명 오영호 ; Oh, Young-Ho
발행사항 [서울 : 한국과학기술원, 1988].
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소장정보

등록번호

4105058

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 8817

SMS전송

도서상태

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초록정보

A study on the fructose production by immobilized whole cell glucose isomerase (GI) was performed in two kinds of hollow fiber reactors; dual hollow fiber reactor and dual circuit hollow fiber reactor. Cell immobilization was accomplished by growing $\underline{Arthrobacter}$ sp. cells possessing GI activity in the reactor. Feeding GI production medium and air to the cells immobilized in the reactor increased the cell biomass and as a result the enzyme activity. After biomass reaching a desired density, the cells were heat treated in-situ and the reactor was used as an enzyme reactor for conversion of glucose to fructose. The final cell mass in dual hollow fiber reactor after 6 days culture was 105g DCW/1. In enzyme reaction of dual hollow fiber reactor which was operated at substrate pH 9.0, temperature of $65\circ{C}$, and substrate flow rate of 4.0ml/h, the enzyme deactivation constant was $1.10\times 10^{-2}l/h$ and the average volumetric fructose productivity for 4 days was 7.61g/l h. This productivity was higher than any other productivities obtained at other operating conditions. At a lower flow rate the substrate flow distribution in the hollow fibers was not good, which resulted in lower fructose productivity. The modeling of hollow fiber enzyme reactor was done. Computer simulation with these modeling equations with $D_3$ value of $0.78\times 10^{-6}cm^2$/s gave result which fitted well the experimental data at a flow rate higher than 2ml/hr. The cell loading in the dual circuit hollow fiber reactor using 35 hollow fibers for air and liquid substrate circuits was 62 gDCW/l after 6 days culture. This is much lower than that of dual hollow fiber reactor As a result the productivity in dual circuit hollow fiber reactor was lower than that of dual hollow fiber reactor. The reason for this poor cell loading was that in cell culture period many cells leaked into the effluent and most of the cells were located at the bottom of the reactor because the space between the fibers was too large to retain the cells.

서지기타정보

서지기타정보
청구기호 {MCE 8817
형태사항 vii, 79 p. : 삽도 ; 26 cm
언어 한국어
일반주기 부록 수록
저자명의 영문표기 : Young-Ho Oh
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 68-71
주제 Bioreactors.
고정화 세포. --과학기술용어시소러스
생물학적 생산. --과학기술용어시소러스
Immobilized cells.
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