The binding of 6-p-touidine-2-naphthalene sulfonate and auramine O to isotactice-poly (2-hydroxyethyl methacrylate) was studied to obtain information on the nature of the interaction between the PHEMA and the dye by equilibrium dialysis, fluorometry and viscometry in the range of 5 - 35℃. The first binding constant and the thermodynamic parameter in the equilibrium dialysis experiments have been calculated. It was found that the free energy change is negative and the enthalpy and the entropy change are largely positive. The results obtained above show that hydrophobic interaction is predominant in the binding of auramine O and TNS by isotactic PHEMA. Temperature dependence of fluorescence intensity (dI/dT) of auramine O in the various salts solution of isotactic PHEMA are also investigated. In the water structure-breaking salt solutions of isotactic PHEMA, Temperature dependence of the fluorescence intensity of auramine O dI/dT is decreased as the ionic B coefficient is decreased. These results can be interpreted in terms of the concept that water structure-breaking salt reduces the structure of the aqueous environment and hence lowers the formation of hydrophobic interactions.
TNS 그리고 auramine O가 isotactic PHEMA와 결합할때 isotactic PHEMA와 염료사이의 결합관계를 알기위하여 5-35℃ 범위에서 equilibrium dialysis, 형광, 점도실험을 하였다. 1차 결합상수와 열역학적인 parameter 가 실험을 통하여 얻어졌다.
결합과정에서 자유에너지는 감소하였고, 엔트로피는 엔탈피와 함께 증가하였다. 이러한 결과로서 isotactic PHEMA 가 auramine O 혹TNS와 결합할때 소수성 결합이 전체결합력에서 지배적인 결합력 임을 알 수 있었다.
Isotactic PHEMA 용액에서 염에 따른 형광도의 온도에 대한 의존도를 연구하였다. Isotactic PHEMA 용액안에 물의 구조를 깨드리는 염이 있 때 그 염의 ionic B coefficient가 작음에 따라서 auramine O 형광도의 온도의존율은 작았다. 이 결과는 염이 수용액의 구조화된 물의 구조를 깨뜨려서 소수성을 감소시키기 때문이다.
