서지주요정보
S.lipolytica 에 의한 구연산 발효와 생물반응기 조업 = Fermentation and bioreactor operation for citric acid production by S.lipolytica
서명 / 저자 S.lipolytica 에 의한 구연산 발효와 생물반응기 조업 = Fermentation and bioreactor operation for citric acid production by S.lipolytica / 조대철.
저자명 조대철 ; Cho, Dea-Chul
발행사항 [서울 : 한국과학기술원, 1987].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

4104635

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 8728

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

A study on the citric acid production using $\underline{S}. \underline{lipolytica}$ was carried out in shake-flask, bubble column, dual hollow fiber bioreactor (DHFBR) and membrane cell recycle reactor. In the immobilized cell culture system the optimum concentration of yeast extract as a nitrogen source was about 5 g/L. Substrate inhibition occurred above 8% (W/V) glucose concentration. Use of 1% $CaCO_3$ as a neutralizing agent greatly enhanced the production of citric acid (40-50 g/L). Repeated batch fermentation for re-use of the cells and long-term operation was carried out and showed higher yield (54%) than any other flask culture. The batch operation in a bubble column showed 63% yield using nitrogen-deficient medium (NDM). In a dual hollow fiber bioreactor operation the volumetric productivity of citric acid (4.55 g/L·h) was superior to those of other reactors (4-20 fold). Use of pure oxygen in stead of air increased the citric acid concentration, the yield and the volumetric productivity. In a membrane cell recycle reactor operation maximum dry cell mass concentration was 39g/L at dilution rate of 0.02 $h^{-1}$ and the yield (69%) with NDM was higher than that (23-38%) with nitrogen-including medium (NIM).

서지기타정보

서지기타정보
청구기호 {MCE 8728
형태사항 vi, 58 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Dea-Chul Cho
지도교수의 한글표기 : 김성철
지도교수의 영문표기 : Sung-Chul Kim
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 56-58
주제 Fermentation.
생물학. --과학기술용어시소러스
발효. --과학기술용어시소러스
Bioreactors.
QR CODE qr code