서지주요정보
트립 오페론 재조합 변이주를 사용한 고농도 세포배양과 플라스미드 안정성 = High density cell culture and the plasmid stability by using TRP-operon r-DNA E.coli
서명 / 저자 트립 오페론 재조합 변이주를 사용한 고농도 세포배양과 플라스미드 안정성 = High density cell culture and the plasmid stability by using TRP-operon r-DNA E.coli / 구만복.
저자명 구만복 ; Gu, Man-Bock
발행사항 [서울 : 한국과학기술원, 1986].
Online Access 원문보기 원문인쇄

소장정보

등록번호

4103600

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 8602

휴대폰 전송

도서상태

이용가능

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반납예정일

초록정보

This study was carried out for the high density cultivation of r-DNA E. coli cells harboring trp plasmid gene in fed-batch and cell filtration recycle system. In batch and fed-batch mode, the cell mass density of 5-10 g/l was obtained and the cells did not grow above it. This may be caused by the inhibitory metabolites produced by the cells. Among those the acetic acid was considered as the most inhibitory material in cell growth. To reduce the inhibitory effect of those toxic materials, the cell filtration recycle system using the hollow fiber membrane module was used. In this mode the cell mass density of 25 g/l was obtained. The stability of this r-DNA E. coli cells in this mode was greatly different from that in shake flask culture repeated. After 6-7 generation 80-90% of total cells in broth was stable in shake flask, but in this mode only 40-50% was stable.

서지기타정보

서지기타정보
청구기호 {MCE 8602
형태사항 v, 63 p. : 삽도 ; 26 cm
언어 한국어
일반주기 Appendix : Standaed curve of tryptophan result of tooth-pick method for plasmid stability
저자명의 영문표기 : Man-Bock Gu
지도교수의 한글표기 : 장호남
공동교수의 한글표기 : 이원국
지도교수의 영문표기 : Ho-Nam Chang
공동교수의 영문표기 : Won-Kook Lee
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 53-61
주제 Plasmid.
세포 배양. --과학기술용어시소러스
플라스미드. --과학기술용어시소러스
Cell culture.
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