서지주요정보
Production of fructose by enzymatic process = 효소공정에 의한 후락토스 생성에 관한 논문
서명 / 저자 Production of fructose by enzymatic process = 효소공정에 의한 후락토스 생성에 관한 논문 / Sung-Won Paik.
저자명 Paik, Sung-Won ; 백성원
발행사항 [서울 : 한국과학기술원, 1976].
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등록번호

4000142

소장위치/청구기호

학술문화관(문화관) 보존서고

MBE 7609

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초록정보

Two strains S-P-1 and S-P-2, both $\underline{Streptomyces}$ $\underline{Sp}$., were found to have relatively high specific enzyme activity compared to that of organisms reported by others. The specific activity of the enzymes produced from these two strains were 0.25 and 0.198 international units respectively. Glucose isomerase from S-P-1 strain was found to be stable under the temperature of heat treatment (at 65℃) for fixation of enzyme inside the Cell. In addition, it was found that this enzyme needed no toxic metallic ion for enzyme activity and xylan could replace xylose as an inducer. Main drawback of this enzyme is that this enzyme had a relatively lower conversion(42.7% fructose) compared to other enzymes, and it had a lower specific activity when xylan was used as an inducer in place of xylose by a factor of 1/15. Optimal temperature and pH of enzymatic reaction was at 85℃ and 7.5 respectively. Reaction kinetics of whole-cell-enzyme was studied and kinetic parameters were determened for the purpose of using these data for operation and designing of enzyme reactor system. The reaction mechanism was found to follow the single substrate reversible reaction kinetics. The kinetic constants determened experimentally are: Kmf = 0.33M, Kmb = 1.0M, Vmf = 0.88 μmole per min., Vmb = 2.96 μmole per min. and Keq = 0.74.

서지기타정보

서지기타정보
청구기호 {MBE 7609
형태사항 [iii], 44 p. : 삽도 ; 27 cm
언어 영어
일반주기 저자명의 한글표기 : 백성원
지도교수의 영문표기 : D.Y. Ryu
지도교수의 한글표기 : 유두영
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 Reference : p. [42]-44
주제 Enzyme kinetics.
Fructose.
Bioreactors.
글루코오스 이성화효소. --과학기술용어시소러스
효소 반응 속도론. --과학기술용어시소러스
프룩토오스. --과학기술용어시소러스
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