서지주요정보
막 재순환 반응기에 의한 페니실린 아실라제 생산 대장균의 고농도 배양 = High cell density culture of E. coli producing penicillin acylase in a membrane cell recycle reactor
서명 / 저자 막 재순환 반응기에 의한 페니실린 아실라제 생산 대장균의 고농도 배양 = High cell density culture of E. coli producing penicillin acylase in a membrane cell recycle reactor / 이율락.
저자명 이율락 ; Lee, Youl-Lark
발행사항 [서울 : 한국과학기술원, 1989].
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소장정보

등록번호

4105472

소장위치/청구기호

학술문화관(문화관) 보존서고

DCE 8906

SMS전송

도서상태

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반납예정일

초록정보

A recombinant $\underline{E}$. $\underline{coli}$ HB101(pPAKS2) producing penicillin acylase was cultured in a membrane cell recycle reactor. Cell density could be increased up to 145 g/L without experiencing by-product inhibitions. The main inhibitory by-product was acetic acid, and cell growth ceased when its concentration was above 14 g/L. Glucose limitation and dissolved oxygen control were indispensable conditions for minimizing acetic acid formation. Acetic acid accumulation was less than 0.5 g/l when cells were cultured with medium containing no glucose. $\underline{E}$. $\underline{coli}$ HB101(pPAKS2) was very stable throughout the whole experiment. Growth retardation at high cell density was caused by inhibitory factors such as acetic acid, carbon dioxide, and osmotic pressure rather than by cell-cell interaction. Use of high concentration medium greatly reduced the membrane surface area required. The recycle reactor could be operated in various operational modes including partial bleed and repeated recycle culture. Productivity of repeated recycle system was over ten times higher than that of a simple batch system. Culture time could be shortened by temperature shift during one batch operation. A mutant $\underline{E}$. $\underline{coli}$ (ATCC 11105) could be also cultured in high cell density. Acetic acid accumulation was less than 0.5 g/L because it was cultured with medium containing no glucose. From the above fact that glucose limitation and dissolved oxygen control were indispensable conditions for the minimization of acetic acid formation, continuous culture was tried with high concentration media, where glucose could be naturally limited. $\underline{E}$. $\underline{coli}$ HB101(pPAKS2) and $\underline{E}$. $\underline{coli}$ (ATCC 11105) were cultured. Using pure oxygen, high cell density up to 95 g/L was obtained without significant inhibition by the main by-product, acetic acid. The operation was simple and productivity was several times higher than those of conventional batch and continuous culture. Dissolved oxygen level and carbon dioxide concentration were important variables.

서지기타정보

서지기타정보
청구기호 {DCE 8906
형태사항 viii, 136 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Youl-Lark Lee
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(박사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 123-132
주제 Escherichia.
Cell culture.
막 반응기. --과학기술용어시소러스
대장균. --과학기술용어시소러스
세포 배양. --과학기술용어시소러스
Membrane reactors.
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