서지주요정보
고정화 미생물에 의한 아크릴아마이드의 생물학적생산 = Biological production of acrylamide by immobilized brevibacterium CH1 cells
서명 / 저자 고정화 미생물에 의한 아크릴아마이드의 생물학적생산 = Biological production of acrylamide by immobilized brevibacterium CH1 cells / 황준식.
저자명 황준식 ; Hwang, Jun-Sik
발행사항 [서울 : 한국과학기술원, 1988].
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4105293

소장위치/청구기호

학술문화관(문화관) 보존서고

DCE 8804

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초록정보

A number of microorganisms are known to possess capability of converting nitrile compounds to the corresponding amides. A bacterial strain of Brevibacterium sp. CH1 has been isolated and used to produce an enzyme necessary for carrying out the reaction of acrylonitrile to acrylamide. Chapter 1 summarizes the commercial use of acrylamide and its current production methods. In chapter 2 screening and identification of Brevibacterium sp. CH1 are discussed. The culture and reaction conditions, and medium optimization were studied for the strain. The conversion yield was nearly 100% with a trace amount of acrylic acid produced. The strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of the amidase toward acrylamide. The substrate specificity o the nitrile hydratase was investigated in a whole cell system. Chapter 3 discusses the cell cultivation in batch and fed-batch system. pH control was very important to obtain high density cell culture and high specific enzyme activity. The maximum growth rate was obtained at a glucose concentration of 20 g/L because of substrate inhibition. For this purpose continuous feeding of complex media was more profitable than that of only glucose medium. The maximum cell density of fed-batch culrure was 68 g/L and the growth yield on cabon source was 0.5. In chapter 4 the effect of different compounds on the enzyme activity of the nitrile-hydratase used for the bioconversion of nitrile was described. An excess of acrylonitrile as a substrate was shown to inhibit the enzyme activity. This inhibition occured only at relatively high substrate concentrations (0.2mol/L or more). The nitrile - bioconversion product (acrylamide, propionamide) and their structural analogues (acrylic acid) were also shown to inhibit the enzyme competitively. The most serious inhibitor found was that of cyanide (Ki=3.78 × 10 mol/L), a break down product of some nitriles. In chapter 5 are discussed continuous production of acrylamide from acrylonitrile using Brevibacterium sp. CHl grown and immobilized in a dual hollow fiber bioreactor. The biomass reached as high as 200g/L based on the space available for the cell growth. The volumetric productivity of the reactor was 88g/L/h and the conversion of acrylonitrile varied with acrylonitrile concentration, pH, temperature and feed rate. Chapter 6 discusses production of acrylamide by biotransformation of acrylonitrile to acrylamide using immobilized whole cell of Brevibacterium sp. CHl in a recycle fed-batch reactor. Acrylamide beads were found to be the best carriers tested in terms of stability and physicochemical strength. For a high final acrylamide concentration, the acrylonitrile concentration and the operational temperature were below 3%, 4℃ respectively, based on maximum acrylamide productivity per unit cell weight and restriction of polymerization.

서지기타정보

서지기타정보
청구기호 {DCE 8804
형태사항 xi, 153 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Jun-Sik Hwang
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(박사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 140-149
주제 Immobilized cells.
Microorganics.
Nitriles.
아크릴아미드. --과학기술용어시소러스
고정화 세포. --과학기술용어시소러스
미생물. --과학기술용어시소러스
니트릴. --과학기술용어시소러스
Acrylamide.
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