서지주요정보
막 재순환 생물반응기에서의 효모와 재조합 대장균의 고농도 배양 = High density cell culture of S.cerevisiae and recombinant E. coli in a membrane cell recycle fermenter
서명 / 저자 막 재순환 생물반응기에서의 효모와 재조합 대장균의 고농도 배양 = High density cell culture of S.cerevisiae and recombinant E. coli in a membrane cell recycle fermenter / 이창우.
저자명 이창우 ; Lee, Chang-Woo
발행사항 [서울 : 한국과학기술원, 1987].
Online Access 원문보기 원문인쇄

소장정보

등록번호

4104148

소장위치/청구기호

학술문화관(문화관) 보존서고

DCE 8703

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

High density cell culture study of microorganisms was carried out using a membrane cell recycle fermenter. The hollow fiber membrane filter is attached to the stirred fermenter and the cell broth is recirculated through the filter unit. The product is continuously removed as filtrate and the cells are recycled to the fermenter. Filter performance is important in this system and is highly dependent on linear velocity along the hollow fiber. The ethanol fermentation by Saccharomyces cerevisiae was carried out. Maximum biomass concentrations up to 210 g-dry wt/L were obtained, but in normal operation concentrations were between 100 and 150 g/L. The ethanol productivity was 85 g/L.h, with an ethanol concentration of 65 g/L and an ethanol yield over 90%. The ethanol productivity and yeast growth rate decreased as the cell concentration increased beyond a certain level. The cell mass in the reactor was maintained by a proper manipulation of dilution rate and bleed ratio depending on the growth rate. Escherichia coli haboring recombinant plasmid and having high tryptophan production activity was cultivated in this system. The maximum cell concentration increased tenfold compared to that in conventional batch process. The main inhibitory metabolic product was acetic acid, which accumulated to 13.5 g/L and inhibited the cell growth. The volumetric productivity of tryptophan was about 0.085 g/L.h. The stability of recombinant rapidly declined as the cell concentration increased during the recycle operation. The wild type E. coli was also cultivated. No metabolic fatty acid accumulated and the cell concentration reached to 95 g/L.

서지기타정보

서지기타정보
청구기호 {DCE 8703
형태사항 vii, 141, [3] p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Chang-Woo Lee
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(박사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 130-141
주제 Fermentation.
Cell culture.
Microorganism.
막. --과학기술용어시소러스
발효. --과학기술용어시소러스
미생물. --과학기술용어시소러스
Membranes.
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