서지주요정보
Zymomonas mobilis의 고정화를 이용한 에탄올 추출 발효에 관한 연구 = Extractive ethanol fermentation using Ca-alginate immobiliaed zymomonas mobilis
서명 / 저자 Zymomonas mobilis의 고정화를 이용한 에탄올 추출 발효에 관한 연구 = Extractive ethanol fermentation using Ca-alginate immobiliaed zymomonas mobilis / 최관영.
저자명 최관영 ; Choi, Kwan-Young
발행사항 [대전 : 한국과학기술원, 1992].
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소장정보

등록번호

8003033

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 92039

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Optimum pH and temperature are determined in an extractive fermentation system using Ca-alginate entrapped Zymonomas mobilis, and some solvents's productivity and toxicity on the free cells and immobilized cells are tested. To increase ethanol productivity in the product inhibition system, an extractive fermentation method combined with ethanol fermentation was applied to the immobilized fermentor. Immobilization is one of the methods to utilize enzymes or microorganisms effectively. It holds many advantages, while some problems remain. Mass transfer resistance in supporting materials like gel is one of the problems. Overall reaction rate of immobilized cell can be estimated by regarding it as a homogeneous system. Extractive fermentation is shown to greatly improve the performance of Zymomonas mobilis in batch and continuous cultures during the conversion of concentrated substrates to ethanol, and it is also used to eliminate the oscillatory behavior often exhibited by z. mobilis in conventional fermentations. As ethanol concentration increased, the distribution coefficients of each solvent increased, the same manner in temperature decrease and pH increase. When the glucose concentration was 200 g/L, 30℃, pH 6.0 and the Oleyl alcohol/medium was 1, extractive fermentation present higher productivity and final ethanol concentration, 5.75 g/L·hr and 89 g/L, respectively than their counterparts, 4.3 g/L·hr and 76 g/L, in general fermentation. The reason for this enhancement is that a product inhibition effect is less due to the improved extraction capacity of the solvent at pH 6.0 and the immobilized cells have relatively constant activity over pH range. Sustained oscillations of biomass, substrate and product concentrations are routinely observed in continuous cultures with Zymomonas. The strong fluctuations of cell viability observed during Zymomonas cultures suggests that viability is a major parameter which unfortunately has never been completely taken into account. If a structuration of the cell population as viable, dead, and non viable cells (unable to divide but still able to produce ethanol) is introduced into a simple mathematical model, any situation from completly stable to completly unstable continuous cultures can be described. Experimental observations suggest that this approach may represent the biological reality more closely than the structured model proposed by Jobses et al.(1985-1987).

서지기타정보

서지기타정보
청구기호 {MCE 92039
형태사항 xi, 108 p. : 삽도 ; 26 cm
언어 한국어
일반주기 부록 수록
저자명의 영문표기 : Kwan-Yong Choi
지도교수의 한글표기 : 양지원
지도교수의 영문표기 : Ji-Won Yang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 90-94
주제 Immobilized cells.
발효. --과학기술용어시소러스
고정화 세포. --과학기술용어시소러스
Alginates.
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