서지주요정보
리파제 생산 균주 Rhizopus chinensis 의 polyurethane foam을 이용한 고정화 = Immobilization of rhizopus chinensis using polyurethane foams
서명 / 저자 리파제 생산 균주 Rhizopus chinensis 의 polyurethane foam을 이용한 고정화 = Immobilization of rhizopus chinensis using polyurethane foams / 주지선.
저자명 주지선 ; Joo, Ji-Sun
발행사항 [대전 : 한국과학기술원, 1992].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

8003032

소장위치/청구기호

학술문화관(문화관) 보존서고

MCE 92038

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

A simple and effective method has been developed for the immobilization of lipase producing Rhizoupus chinensis on polyurethane foam. In this method the fungal cells with 1-3 specific lipase in their inside are immobilized within the foam matrix. Four types of commercially available polyurethane foam were tested. The ultimate purpose of the process is to produce low-cost biocatalysts for lipase-catalyzed reactions which are being increasingly used for industrial applications. Effects of several parameters were studied on the cell loading and the hydrolytic activity of intracellular lipase after acetone drying. These parameters were the type, size, and amount of polyurethane foam. In all the cases the intracellular lipase activity obtained with the foam was approximately twice greater than that obtained in the absence of the foam. For the hydrolysis test an olive oil emulsion was used as the feed. Effects of pH, temperature and the concentration of $CaCl_2$ were also investigated on the hydrolytic activity. It was observed that the optimum pH, temperature, and $CaCl_2$ concentration were 7.4, 37℃, and 0.027 (w/v %), repectively.

서지기타정보

서지기타정보
청구기호 {MCE 92038
형태사항 vi, 56 p. : 삽도 ; 26 cm
언어 한국어
일반주기 부록 수록
저자명의 영문표기 : Ji-Sun Joo
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 51-54
주제 Polyurethanes.
고정화 세포. --과학기술용어시소러스
폴리우레탄. --과학기술용어시소러스
Immobilized cells.
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