서지주요정보
전자현미경을 이용한 폴리라이신 분자량과 농도에 따른 튜불린의 자기조립구조 연구 = TEM study of tubulin and polylysine assembly:molecular weight and concentration dependence
서명 / 저자 전자현미경을 이용한 폴리라이신 분자량과 농도에 따른 튜불린의 자기조립구조 연구 = TEM study of tubulin and polylysine assembly:molecular weight and concentration dependence / 김유미.
저자명 김유미 ; Kim, Yu-Mi
발행사항 [대전 : 한국과학기술원, 2014].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8026293

소장위치/청구기호

학술문화관(문화관) 보존서고

MBIS 14002

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Microtubules (MTs) are 25 nm protein nanotubes with wall comprised of protofilaments built from αβ-tubulin and have a negative charge (surface charge density ? -0.87 e/nm2). MTs are involved in cell division, intracellular trafficking, establishment and maintenance of cell shape. The previous studies show that tubulin with multivalent cations/ cationic polymer complex forms ring and tubular structure without GTP according to charge interaction. In order to control these structure, by using transmission electron microscopy (TEM) we used polylysine (surface charge density ? 2.12 e/nm2) and observed the effect of polylysine molecular weight, concentration (R = concentration of polylysine/concentration of tubulin) and Taxol on the assembly structure of tubulin and polylysine. Assembled with polylysine, tubulin forms various structures which includes the single protofilament ring (SPR), double protofilament ring (DPR), tubulin sheet, bundle of protofilaments (PFs), single walled tubulin tube (SWTT) and double walled tubulin tube (DWTT). For 500 - 2000 Da polylysine, TEM show DPR at R = 0.25 and DWTT at R ?? 0.5. MT like, PFs bundle, tubulin sheet at R = 0.05 - 0.25 and DWTT at R ≥ 0.375 are observed in the presence of Taxol. For 150k - 300k Da, TEM show DPR, short DWTT at R = 0.1 - 0.2, short PFs at R = 0.2 and SPR, SWTT at R ≥ 0.25. MT like, PFs bundle, tubulin sheet at R = 0.01 - 0.05, short DWTT at R = 0.1 - 0.25, SWTT at R ≥ 0.25 are observed in the presence of Taxol. PEGylated-polylysine (PEG-PL) has been used, resulting in similar assembly structures. DPR, SPR, DWTT and SWTT are observed at higher R than the case of polylysine. PEG-PL prevent polylysine induced aggregation of tubulin tubes. This study contributes to quantify nano structure using x-ray scattering and provide an insight into how we can functionalize tubulin based nanotubes for drug delivery system.

서지기타정보

서지기타정보
청구기호 {MBIS 14002
형태사항 v, 51 p. : 삽도 ; 30 cm
언어 한국어
일반주기 저자명의 영문표기 : Yu-Mi Kim
지도교수의 한글표기 : 최명철
지도교수의 영문표기 : Myung-Chul Choi
학위논문 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과,
서지주기 참고문헌 : p. 49-51
주제 튜불린
마이크로튜불
Tubulin
Microtubule
nano tube
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