서지주요정보
An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231 = An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231
서명 / 저자 An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231 = An investigation of tropomyosin 4 in the regulation of invadopodia and motility in MDA-MB-231 / Sabina Kim.
저자명 Kim, Sabina ; Kim, Sabina
발행사항 [대전 : 한국과학기술원, 2013].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8025605

소장위치/청구기호

학술문화관(문화관) 보존서고

MNST 13001

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Tropomyosins are α-helical coiled coil proteins that bind actin filaments. Four genes encode the tropomyosin family of proteins that includes about 40 members. Studies of tropomyosins revealed a functional diversity between different isoforms. TPM4 gene encodes 2 isoforms: tropomyosin 4 low molecular weight (TM4 LMW) and tropomyosin 4 high molecular weight (TM4 HMW). TM4 LMW has been reported to regulate resorptive capacity of osteoclasts. However, the role of TM4 in facilitating degradation and motility in cancer cells has not been investigated yet. In this research, I used siRNAs to TM4 LMW and TM4 HMW and performed degradation and migration assays in MDA-MB-231 breast cancer cells to study the role of both isoforms. I found that degradation is regulated by TM4 LMW, but not TM4 HMW. This is first evidence that TM4 might regulate invadopodia dynamics in tumor cells. In addition, this result might indicate that TM4 isoforms don’t possess a redundant function. Further research should focus on mechanism underlying the observed effect by TM4 LMW. In conclusion, this study contributes to our knowledge regarding invadopodia and the role of cytoskeletal proteins in the regulation of these structures.

Tropomyosins are α-helical coiled coil proteins that bind actin filaments. Four genes encode the tropomyosin family of proteins that includes about 40 members. Studies of tropomyosins revealed a functional diversity between different isoforms. TPM4 gene encodes 2 isoforms: tropomyosin 4 low molecular weight (TM4 LMW) and tropomyosin 4 high molecular weight (TM4 HMW). TM4 LMW has been reported to regulate resorptive capacity of osteoclasts. However, the role of TM4 in facilitating degradation and motility in cancer cells has not been investigated yet. In this research, I used siRNAs to TM4 LMW and TM4 HMW and performed degradation and migration assays in MDA-MB-231 breast cancer cells to study the role of both isoforms. I found that degradation is regulated by TM4 LMW, but not TM4 HMW. This is first evidence that TM4 might regulate invadopodia dynamics in tumor cells. In addition, this result might indicate that TM4 isoforms don’t possess a redundant function. Further research should focus on mechanism underlying the observed effect by TM4 LMW. In conclusion, this study contributes to our knowledge regarding invadopodia and the role of cytoskeletal proteins in the regulation of these structures.

서지기타정보

서지기타정보
청구기호 {MNST 13001
형태사항 iii, 28 p. : 삽도 ; 30 cm
언어 영어
일반주기 저자명의 한글표기 : Sabina Kim
지도교수의 영문표기 : David M. Helfman
지도교수의 한글표기 : 데이비드 헬프만
학위논문 학위논문(석사) - 한국과학기술원 : 나노과학기술학과,
서지주기 References : p. 24-27
주제 invadopodia
tropomyosin
degradation
migration
invadopodia
tropomyosin
degradation
migration
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