An ECL(electrochemiluminescence) chip for bio molecule analysis using autonomous microbead positioning has been proposed. The chip for the detection of ECL of Tris(2,2’-bipyridyl)ruthenium(II) [$Ru(bpy)_3^{2+}$] has been microfabricated in silicon and designed for use in the immunoassay. The chip is a vertical assembly of micromachined silicon and PDMS, containing a gold, thin film electrode. The PDMS channel is transparent, enabling the detection of the luminescence by an external PMT(photomultiplier tube). Preliminary tests have yielded the detection of [$Ru(bpy)_3^{2+}$] concentrations from $10^{-6}M$ to $10^{-4}M$ with a chip volume of 200pl.
