서지주요정보
재조합 Saccharomyces cerevisiae의 유가식 배양에 의한 monellin 생산 최적화 연구 = A study on the optimization of monellin production by fed-batch culture of a recombinant Saccharomyces cerevisiae
서명 / 저자 재조합 Saccharomyces cerevisiae의 유가식 배양에 의한 monellin 생산 최적화 연구 = A study on the optimization of monellin production by fed-batch culture of a recombinant Saccharomyces cerevisiae / 전혜경.
저자명 전혜경 ; Chon, Hye-Kyoung
단체명 생명화학공학과
발행사항 [대전 : 한국과학기술원, 2002].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8012971

소장위치/청구기호

학술문화관(문화관) 보존서고

MCBE 02023

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Monellin is a protein known to have a very high specificity for the human sweet taste receptor. It has been isolated from African berries and is approximately 100,000 times sweeter than sucrose on a molar basis. In this study, fed-batch cultures for the overproduction of monellin using recombinant Saccharomyces cerevisiae, which contains a glucose repressible ADH2/GAP(alcohol dehydrogenase 2/ glyceraldehyde 3-phosphate dehydrogenase) hybrid promoter, were carried out at pH 5.0 for 48 hours with constant feed rates from 0.10 to 0.50mL/min. The feed glucose concentration was 370 g/L. The best result was obtained when the feed rate was 0.14 mL/min with a monellin concentration of 188.64 mg/L. Two stage fed cultures were also carried out with the feeding of glucose at 0.14 mL/min and a secondary feeding of ethanol in the second stage. When ethanol feeding was started with the glucose feeding ceased at 35 hours, the highest monellin concentration of 289.36 mg/L was obtained. Another series of fed-batch cultures with the feed rate fixed at 0.14 mL/min were carried out for five different pH`s, that is, 3.5, 4.0, 4.5, 5.0 and 6.0. The highest monellin concentration of 252.75 mg/L was obtained at pH 4.0. The two stage culture was repeated at pH 4.0 expecting a synergy effect of the secondary feeding of ethanol and the optimized pH. But, no significant synergy effect was observed. A further study on the simultaneous optimization of the pH and feeding strategy is in need.

서지기타정보

서지기타정보
청구기호 {MCBE 02023
형태사항 v, 65 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hye-Kyoung Chon
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학과명칭변경 : 화학공학과가 생명화학공학과로 변경됨
학위논문 학위논문(석사) - 한국과학기술원 : 생명화학공학과,
서지주기 참고문헌 : p. 64-65
주제 유가식 배양
재조합
효모
모넬린
최적화
fed-batch
recombinant
Saccharomyces
cerevisiae
monellin
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