서지주요정보
광면역 센서를 위한 항체 고정화에 관한 연구 = A study on antibody immobilization for immuno-optical sensors
서명 / 저자 광면역 센서를 위한 항체 고정화에 관한 연구 = A study on antibody immobilization for immuno-optical sensors / 정현주.
저자명 정현주 ; Jung, Hyun-Ju
발행사항 [대전 : 한국과학기술원, 2001].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8011857

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 01028

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

An experimental study was carried out on the immobilization of antibody via two methods, the covalent binding and the avidin-biotin binding, and on the effect of two blocking agents, bovine serum albumin and ethanolamine. In the covalent binding method, a slide glass was treated with 3-mercaptopropyl-trimethoxysilane (MTS) and γ-maleimidobutyric acid N-hydroxysuccinimide ester (GMBS), then the antibody, Anti-Mouse IgG was covalently attached to GMBS. In the avidin biotin binding method, avidin was attached to GMBS then biotinylated Anti-Mouse IgG was attached to avidin. In both methods, the amount of immobilized antibody increased with increasing antibody concentration in the solution added. However, it was not exactly proportional to the antibody concentration showing a saturated form. When the avidin-biotin binding method was used, about 3 times larger amount of the antibody was immobilized than the covalent binding method. Bovine serum albumin(BSA) was more effective as blocking agent than ethanolamine(EA) in the covalent binding method, while EA was more effective than BSA in the avidin-biotin method.

서지기타정보

서지기타정보
청구기호 {MCHE 01028
형태사항 [vii], 48 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hyun-Ju Jung
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 47-48
주제 항체 고정화
광면역센서
비특이적 흡착 방지
Antibody immobilization
Immuno-optical sensors
blocking agent
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