서지주요정보
재조합 Sacchromyces cerevisiae의 유가식 배양에 의한 외래단백질 생산의 최적화 연구 = A study on the production of foreign protein by fed-batch of a recombinant Saccharomyces cerevisiae
서명 / 저자 재조합 Sacchromyces cerevisiae의 유가식 배양에 의한 외래단백질 생산의 최적화 연구 = A study on the production of foreign protein by fed-batch of a recombinant Saccharomyces cerevisiae / 이태화.
저자명 이태화 ; Lee, Tae-Hwa
발행사항 [대전 : 한국과학기술원, 2001].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8011852

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 01023

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

In this study, the optimization of two-stage fed-batch culture for the production of a foreign protein using recombinant Saccharomyces cerevisiae containing the glucose repressible ADH2/GAP (alcohol dehydrogenase2/glyceraldehyde 3-phosphate dehydrogenase) hybrid promoter was carried out. In the first stage of cell growth on glucose, constant, exponential and ethanol-concentration-control feeding strategies were employed. In the second stage of protein production, ethanol was supplied as the carbon source, and the promoter was activated automatically upon the depletion of glucose. The ethanol concentration was kept lower a certain level to minimize its inhibitory effects and thus to maximize gene expression. With an exponential feeding at the specific growth rate higher than $0.10hr^{-1}$ in the first stage, ethanol was produced intensively, and no high-level production of protein could not be achieved in the second stage. When constant feeding strategy was used in the first stage and ethanol was fed intermittently in the second stage to keep its concentration lower than 10g/L, the final concentration of protein was as high as 286mg/L.

서지기타정보

서지기타정보
청구기호 {MCHE 01023
형태사항 iv, 59 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Tae-Hwa Lee
지도교수의 한글표기 : 장용근
지도교수의 영문표기 : Yong-Keun Chang
학위논문 학위논문(석사) - 한국과학기술원: 화학공학과,
서지주기 참고문헌 : p. 57-59
주제 외래단백질
foreign protein
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