서지주요정보
Biological production of succinic acid by anaerobiospirillum succiniciproducens and mannheimia sp. = Anaerobiospirillum succiniciproducens와 mannheimia sp.을 이용한 생물학적 숙신산 생산
서명 / 저자 Biological production of succinic acid by anaerobiospirillum succiniciproducens and mannheimia sp. = Anaerobiospirillum succiniciproducens와 mannheimia sp.을 이용한 생물학적 숙신산 생산 / Pyung-Cheon Lee.
저자명 Lee, Pyung-Cheon ; 이평천
발행사항 [대전 : 한국과학기술원, 2001].
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등록번호

8012327

소장위치/청구기호

학술문화관(문화관) 보존서고

DCHE 01011

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초록정보

Physiological and cultural characteristics of a succinate-producing Anaerobiospirillum succiniciproducens and a novel succinate-producing Mannheimia sp., isolated from bovine rumen, were studied to develop the biological systems for succinic acid production. Among several environmental and physiological factors affecting cell growth and end-product formation, the external supply of $CO_2$ and $H_2$ enhanced the growth of A. succiniciproducens and its succinic acid formation. Steady-state parameters obtained by the continuous cultures of A. succiniciproducens indicated that both succinic acid production and acetic acid production were found to be growth-associated and that the formation of unwanted acetic acid reduced the succinic acid yield and cell growth. Acetic acid formation could be avoided by using glycerol as a carbon source during the batch and continuous cultures. Accordingly, a high yield and productivity of succinic acid could be obtained. Inexpensive materials such as whey, wood hydrolysate, and corn steep liquor could be effectively utilized by A. succiniciproducens. A novel succinate-producing Mannheimia sp. 55E isolated from bovine rumen is a metabolically versatile bacterium capable of growing either anaerobically and aerobically. It fermented glucose to succinic, acetic, formic, and lactic acids in response to environmental conditions. Under 100% $CO_2$ condition, more succinic acid and less lactic acid were formed, and cells grew well. The culture pH did not affect the ratio of end-products formed under 100% $CO_2$condition. Whereas, under 100% $N_2$ condition, more lactic acid and less succinic acid were formed, and cells grew poor. Aerobically, Mannheimia sp. 55E produces lactic and acetic acids without exhausted $CO_2$ gas and irrespective of initial glucose concentration, which indicated that this bacterium may not have tricarboxylic acid cycle (TCA). The pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme in the central metabolic pathway of the metabolically versatile Mannheimia sp. 55E, was cloned, sequenced, and characterized. The gene encoded a 538-residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the Mannheimia sp. 55E PEPCK was similar to those of all known ATP-dependent PEPCKs. Mannheimia sp. 55E effectively utilized whey and corn steep liquor. Consequently, the data obtained in this study provide physiological and genetic information on succinate-producing bacteria in general, and also form the basis for the economical and feasible production of succinic acid from renewable sources.

서지기타정보

서지기타정보
청구기호 {DCHE 01011
형태사항 x, 127 p. : 삽도 ; 26 cm
언어 영어
일반주기 저자명의 한글표기 : 이평천
지도교수의 영문표기 : Ho-Nam Chang
공동교수의 영문표기 : Sang-Yup Lee
지도교수의 한글표기 : 장호남
공동교수의 한글표기 : 이상엽
수록잡지명 : "Batch and continuous fermentation of succinic acid from whey by Anaerobiospirillum succiniciproducens". Applied microbiology and biotechnology, v.54, pp.23-27 (2000)
수록잡지명 : "Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source". Biotechnology and bioengineering, v.72 no.1, pp.41-48 (2001)
학위논문 학위논문(박사) - 한국과학기술원 : 화학공학과,
서지주기 Reference : p. 111-119
주제 Succinic acid
Anaerobiospirillum succiniciproducens
Mannheimia sp.
숙신산
Anaerobiospirillum succiniciproducens
Mannheimia sp.
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