서지주요정보
NADH가 캡슐화된 졸-겔 실리카 겔의 흡광 및 형광 = Optical absorption and fluorescence of NADH encapsulated Sol-Gel silica gel
서명 / 저자 NADH가 캡슐화된 졸-겔 실리카 겔의 흡광 및 형광 = Optical absorption and fluorescence of NADH encapsulated Sol-Gel silica gel / 홍혜정.
저자명 홍혜정 ; Hong, Hye-Joung
발행사항 [대전 : 한국과학기술원, 2000].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8011096

소장위치/청구기호

학술문화관(문화관) 보존서고

MMS 00046

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Sol-gel technique has been applied to encapsulation of biomolecules, such as enzymes and coenzymes, because this method is a low temperature process and obtains a transparent porous matrix. In particular, the matrix prepared from organically substituted silanes may influence the typical chemical activity of organic molecules entrapped. To fabricate enzyme encapsulated sol-gel silica gel, thetramethyl orthosilicate (TMOS), 3-aminopropyltrimethoxysilane (APTMS), and methyltriethoxysilane (MTES) were used as precursors. Alcohol dehydrogenase (ADH) and β-nicotinamide adenine dinucleotide ($NAD^+$) were also used as an enzyme and a coenzyme encapsulated in gel matrix. The reduced form of coenzyme (NADH) absorbs strongly around 340nm and fluoresces with a 440~460nm fluorescent maximum so that the application to an optical biosensor is possible. Thus ADH can be employed as a sensor for alcohol that takes advantage of NADH fluorescence. Using precursors with different functional group, transparent porous glasses in which NADH or $NAD^+$ /ADH were encapsulated were fabricated. Measurements of fluorescence and absorbance of enzyme encapsulated in gel were carried out with a spectrofluorimeter and a UV spectrophotometer, respectively. Thorough measurements, we confirmed that 450nm fluorescence intensity increased by enzyme reaction and enzyme activity was retained in gel. The pore size and microstructure of NADH encapsulated gel were measured using DSC, XRD, SEM, TEM. In summary, the enzyme activity depends on the microstructure of the gel influenced by the process conditions.

서지기타정보

서지기타정보
청구기호 {MMS 00046
형태사항 v, 83 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hye-Joung Hong
지도교수의 한글표기 : 배병수
공동교수의 한글표기 : 김도경
지도교수의 영문표기 : Byeong-Soo Bae
공동교수의 영문표기 : Do-Kyung Kim
학위논문 학위논문(석사) - 한국과학기술원 : 재료공학과,
서지주기 참고문헌 : p. 80-83
주제 졸-겔
니코틴아미드아데닌다이뉴클레오타이드
캡슐화
흡광
형광
Sol-gel
NADH
Encapsulation
Absorption
Fluorescence
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