A micro biosensor has been proposed and fabricated for the measurement of glucose concentration in whole blood. Micro structures including the syringe with a channel and a reaction chamber have been fabricated using Multi-Exposure Single-Development (MESD) method. The electrochemical system for the measurement of glucose concentration is composed of two electrodes: gold electrode (working electrode) and silver electrode (reference electrode). The electropolymerization method has been applied for the immobilization of GOD with polypyrrole on the working electrode. The calibration of the micro electrode array has been characterized for various glucose concentration from 0 mM to 200 mM. These characteristics demonstrate that the fabricated micro biosensors are suitable for the diagnosis of diabetic patients whose glucose level is higher than 8 mM.
