서지주요정보
Halobacterium halobium R1에 의한 박테리오로돕신의 생산 = Production of bacteriorhodopsin by halobacterium halobium R1
서명 / 저자 Halobacterium halobium R1에 의한 박테리오로돕신의 생산 = Production of bacteriorhodopsin by halobacterium halobium R1 / 엄영순.
저자명 엄영순 ; Um, Young-Soon
발행사항 [대전 : 한국과학기술원, 1997].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8007515

소장위치/청구기호

학술문화관(문화관) 보존서고

MCHE 97017

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Bacteriorhodopsin (BR) in the purple membrane from halobacteria has recently been studied to apply the design of molecular electron device and optical computers In this study, the effects of nutrient conditions and growth conditions on the production of bacteriorhodopsin (BR) by Halobacterium halobium R1 were examined. In order to increase the productivity, an internal membrane bioreactor system and an external membrane cell recycle system were employed. In order to supplement amino acids to the medium, various complex nitrogen sources containing amino acids were examined in the flask culture. The yeast extract was found to be the best amino acids source. Among the elements of amino acids, argine, isoleucine, leucine, lysine, methionine and valine were found to be essential for growth. Six different cultures were provided by adding each of the six essential amino acids to the medium containing 5g/L of yeast extract, and the growth behavior of H. halobium R1 was observed. However, any difference in the growth rate was not observed. The halobacteria are reported to utilize proteins and amino acids rather than carbohydrates, except glycerol. Experimental results showed that 0.1~1% glycerol stimulated the growth of cell as well as the production of bacteriorhodopsin. The pH of the culture changed from 7 to 8 during growth. Through the study of the effect of initial pH on cell growth, the culture whose initial pH was 5 or 8 showed longer lag phase. The growth of H. halobium R1 and BR production were found to depend on the dissolved oxygen level rather than the pH value. The BR content of total protein was 1.25% under the aeration condition of 4~5 L air /min(2.7~3.3vvm), and 2.1% under the aeration condition of 2 L air/min(1.3 vvm). In order to remove toxic product for the high cell density fermentation, an internal membrane bioreactor system and an external membrane cell recycle system were employed to obtain high cell density culture. As a result, productivity of 125 mg/L of BR was obtained from the internal membrane bioreactor system at a dilution rate of $0.066 hr^{-1}$, and that of 200~400 mg/L of BR from the external membrane cell recycle system at a dilution rate of $0.1 hr^{-1}$. The productivity achieved by the external membrane cell recycle system was 10~15 fold higher than that obtained by the corresponding batch cultivations.

서지기타정보

서지기타정보
청구기호 {MCHE 97017
형태사항 x, 54 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Young-Soon Um
지도교수의 한글표기 : 장호남
지도교수의 영문표기 : Ho-Nam Chang
학위논문 학위논문(석사) - 한국과학기술원 : 화학공학과,
서지주기 참고문헌 : p. 52-54
주제 할로박테리움 할로비움 R1
박테리오로돕신
Halobacterium halobium R1
Bacteriorhodopsin
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