서지주요정보
L-lactate dehydrogenase 이용한 후막형 전기화학식 L-Lactate 바이오센서의 개발 = Thick-film amperometric L-lactate biosensor using L-lactate dehydrogenase
서명 / 저자 L-lactate dehydrogenase 이용한 후막형 전기화학식 L-Lactate 바이오센서의 개발 = Thick-film amperometric L-lactate biosensor using L-lactate dehydrogenase / 윤현철.
저자명 윤현철 ; Yoon, Hyun-Chul
발행사항 [대전 : 한국과학기술원, 1996].
Online Access 원문보기 원문인쇄

소장정보

등록번호

8006714

소장위치/청구기호

학술문화관(문화관) 보존서고

MBS 96018

SMS전송

도서상태

이용가능

대출가능

반납예정일

초록정보

Biosensors have been attracting much attention as a promising tool to analyze an analyte in the field of diagnosis, environment, food, and bioprocess. However, only a limited number of biosensors has been applied to real samples, and very few are commercialized so far. A prerequisite for commercialization is that the biosensor must be fabricated at least on a medium batch scale with a reasonable cost. Thick-film technology is well known to be valuable for the production of one-shot enzyme electrode due to its high production efficiency. From an analytical point of view, $NAD^+$/NADP - dependent dehydrogenase is regarded as one of the most important enzymes. But these dehydrogenases have not been successfully used for the enzyme sensor in spite of their broad range of catalytic capabilities. Major barrier to the practical application of dehydrogenase lies in the difficulty in regeneration of the coenzyme and poor electrocatalytic behaviors of $NAD^+$/NADH couple. An amperometric biosensor for the determination of L-lactate was fabricated using L-lactate dehydrogenase by means of thick-film technology. The biosensor strip consists of three electrodes: a carbon counter electrode, a psuedo-reference electrode (Ag/AgCl), and a working electrode based on carbon / complex polymer resin / lactate dehydrogenase / $NAD^+$. Voltammetric and amperometric studies on the thick film enzyme electrodes were conducted to evaluate special electrochemical characteristics. Optimal conditions for the preparation and operation of the resulting L-lactate biosensor were evaluated in terms of the sensitivity and stability.

서지기타정보

서지기타정보
청구기호 {MBS 96018
형태사항 v, 50 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Hyun-Chul Yoon
지도교수의 한글표기 : 김학성
지도교수의 영문표기 : Hak-Sung Kim
학위논문 학위논문(석사) - 한국과학기술원 : 생물과학과,
서지주기 참고문헌 : p. 45-48
주제 후막소자기법
전기화학식 바이오센서
L-lactate
L-lactate dehydrogenase
Thick-film technology
Amperometric biosensor
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