서지주요정보
Vaccinia virus와 HeLa S3 숙주세포를 이용한 β-galactosidase의 생산 수율 향상 = Enhancement of β-galactosidase productivity in vaccinia virus/HeLa S3 expression system
서명 / 저자 Vaccinia virus와 HeLa S3 숙주세포를 이용한 β-galactosidase의 생산 수율 향상 = Enhancement of β-galactosidase productivity in vaccinia virus/HeLa S3 expression system / 김병재.
저자명 김병재 ; Kim, Byung-Jae
발행사항 [대전 : 한국과학기술원, 1995].
Online Access 제한공개(로그인 후 원문보기 가능)원문

소장정보

등록번호

8005138

소장위치/청구기호

학술문화관(문화관) 보존서고

MBT 95001

휴대폰 전송

도서상태

이용가능

대출가능

반납예정일

초록정보

The vaccinia virus(vSC 8) expression system was employed for the production of β-galactosldase as a model heterologous protein. HeLa S3 cells were used as a host cell line for their higher production and scale-up potential. The β-galactosldase production could be substantially increased by increasing virus infectivity and host cell activity. The virus infectivity was determined by staining the producing cells using Xgal as substrate. When HeLa S3 cells were infected with 5MOI, the infectivity was usually 20-35%. As infection media, serum, polylysine concentration and buffers were tested for the investigation of the effects on virus infection. The optimal serum concentration and buffer were determined to be 1% of calf serum and HBSS, respectively. The virus infectivity and β-galactosldase production were increased only by 10% and 25% respectively when 2-25mg/L polylysine was added in DMEM containing 2.5% calf serum as infection medium. When HeLa S3 cells were treated with 0.0010.1μM of methotrexate for 24 hours prior to infection, the β-galactosldase production and specific productivity were increased by 2.5 -3 times, respectively.

서지기타정보

서지기타정보
청구기호 {MBT 95001
형태사항 vii, 37 p. : 삽도 ; 26 cm
언어 한국어
일반주기 저자명의 영문표기 : Byung-Jae Kim
지도교수의 한글표기 : 김정회
지도교수의 영문표기 : Jung-Hoe Kim
학위논문 학위논문(석사) - 한국과학기술원 : 생물공학과,
서지주기 참고문헌 : p. 34-35
주제 Gene expression.
Viruses.
유전자 발현. --과학기술용어시소러스
갈락토시다아제. --과학기술용어시소러스
세포 배양. --과학기술용어시소러스
바이러스. --과학기술용어시소러스
Cell culture.
QR CODE qr code