In recent years, an intense interest has grown in the synthesis of functional hybrid organic-inorganic nanostructured materials. Several research groups have reported different hybrid organic-inorganic nanomaterials by employing organic materials (such as proteins, amino acids, and organic acids) and inorganic materials (such as metal ion and metal nanoparticles). Based on the aforementioned unique combinations of organic-inorganic hybrid nanomaterials, we have developed a novel nanostrucuted materials employed in sensing different biomolecules such $H_2O_2$, glucose, choline, and acetyl choline. In chapter 2, we developed a simple but efficient method to synthesize protein-inorganic hybrid nanostructures with a flower-like shape (nanoflowers), which relies on sonication to facilitate the synthesis of the nanoflowers. With this technique, we synthesized nanoflowers containing laccase as a model enzyme and copper phosphate within 5 minutes at room temperature. The resulting laccase nanoflowers yielded greatly enhanced activity, stability, and reusability, and their usefulness was successfully demonstrated by applying them in the colorimetric detection of epinephrine. In chapter 3, we reported sonicated bovine serum albumin (BSA)-incorporated $Cu_3(PO_4)_2.3H_2O$ nanoflowers (SBSA-NFs) possess an intrinsic peroxidase-mimicking activity and also demonstrate that glucose oxidase (GOx) incorporated in nanoflowers showed enhanced activity compared with free GOx for the detection of target glucose in the cascade reaction, due to the synergistic effect arises from improved spatial coupling between enzymes and peroxidase-mimicking copper(II). In chapter 4, we synthesized different amine grafted MIL-100(Fe) metal organic frameworks (MOFs) and found that N,N,N',N' Tetramethyl- 1, 4- butanediamine (TMBDA)-MIL-100(Fe) showed excellent peroxidase-mimicking activity. Further, we developed detection strategy by employing TMBDA-MIL-100(Fe) for the detection of biomolecules and obtained excellent limit of detection for $H_2O_2$, choline, and acetylcholine with $0.329 \mu M$ , $0.027 \mu M$, and $0.036 \mu M$ respectively. The diagnostic capability of this method is also demonstrated by detecting choline and acetyl choline in serum, showing its great potential in practical application.
최근 유기물-무기물 혼합 나노 물질의 합성에 대한 관심이 매우 높아지고 있으며, 실제로, 몇몇 연구 그룹에 의해 유기물 (단백질, 아미노산, 유기산 등)과 무기물 (금속 이온, 금속 나노 입자 등)을 이용한 여러 종류의 유기물-무기물 혼합 나노 물질의 합성에 대한 연구가 활발히 이루어지고 있다. 본 연구에서는 유기물-무기물 혼합 나노 물질을 형성할 수 있는 여러 종류의 물질 조합을 기반으로 하여 새로운 나노 물질을 개발하였으며, 더 나아가, 본 시스템을 과산화수소, 글루코스, 콜린, 아세틸 콜린 등과 같은 생체 분자의 검출에 활용하였다. 제 2 장에서는, 꽃 모양을 가지는 단백질-유기물 혼합 나노 구조의 간편하고 효과적인 합성 기술을 개발하였으며, 이는 라카아제 효소의 고정화에 활용될 수 있음을 규명하였다. 제 3 장에서는, 초음파 처리가 이루어진 글루코스 산화효소-나노꽃 복합체가 가지고 있는 과산화효소 활성을 규명하였으며, 이를 이용하여 글루코스를 검출하였다. 또한, 제 4 장에서는, 아민이 접합된 금속유기구조체가 가지고 있는 과산화효소 활성을 규명하였으며, 이를 과산화수소, 콜린, 아세틸콜린 등의 물질을 검출하는데 적용이 가능하다는 것을 실험적으로 증명하였다.