A new buffer system which induce competent state of $\underline{Z}. \underline{anaerobia}$ as well as $\underline{E}. \underline{coli}$ has been developed. Using the new buffer transformation of $\underline{Z}. \underline{anaerobia}$ with three types of plasmids, i,e, pBR325, pRK25011 and pDH73 were succeeded. The main point of the new buffer system is in the use of $Mn^{++}$ ion and lysozyme. The concentration of lysozyme in the buffer was restricted to 0.4 mg/ml which allowed modification of cell wall structure but remained cells alive.
Among the three systems developed one system which is relatively simple with high efficiency composed as follows; $MnCl_2$-70mM, 30mM-$CaCl_2$, 10mM-$NaCl$, 100mM-$RbCl_2$, 40mM-$LiCl_2$, 50mM-$Cscl$ and 3mM-$Hcocl_3$.
After confirming successful transformation of $\underline{Z}. \underline{anaerobia}$ with pBR325 and pRK 2501, another transformation of the same recipient cells with pDH73 was also tried. The plasmid pDH73 which has been constructed in this laboratory contains CMCase gene of $\underline{B}. \underline{subtilis}$. The recipient organism $\underline{Z}. \underline{anaerobia}$ has been known as an effective ethanol producer. Therefore, the success in transformation of $\underline{Z}. \underline{anaerobia}$ with pDH73 in this research will contribute for the future study on ethanol production of $\underline{Z}. \underline{anaerobia}$ using cellulosic materials significantly.
새로운 형질변환 완충용액을 개발하였다.
그 조성은 망간이온, 여러가지 금속이온들 그리고 라이소자임등의 조합으로 이루어졌다.
이것을 가지고 Zymomonasanaerobia의 형질변환을 시도한 결과 사용한 플라스 미드의 표현형질을 확인할 수 있었다.
특히 섬유소 분해유전자의 형질발현을 볼수있었다.
ColE1 replicon도 Zymomonas anaerobia에서 잘 발현되었다.
