Extracellular inulase which hydrolyze β-2:1' linked fructose polymer, inulin, was prepared from $\mbox{\underline{Saccharomyces}}$ $\mbox{\underline{fragilis}}$ in the artichoke extract media. The enzyme was purified by sepharose 6B gel filtration and DEAE-Sephadex A-50 ion exchange chromatography. The purified enzyme which was confirmed by electrophoresis running at $p^H$ 9.0 and 6.8 was characterized and used for the production of fructose from Jerusalem artichoke ($\mbox{\underline{Helianthns}}$ $\mbox{\underline{tuberosus}}$ $\mbox{\underline{L}}$.) by enzymatic hydrolysis. The enzyme was found although the general properties of the enzyme varied against substrates in terms of $p^H$ and temperature. Oprimal $p^H$ of invertase and inulase activities were 3.0 and 5.2 respectively, while both activities were stable between $p^H$ 5.0 and 7.0 at 30℃. Optimal temperatures of invertase and inulase activities were shown to be 60℃ and 55℃ respectively. At 50℃ both activities were stable about 4 hrs. With the recovery of 95% of original activities. Inulase activity was more stable than invertase activity at 55℃ and 60℃. At 65℃ both activities were almost lost in 15min. The activation energies for hydrolyses of sucrose and inulin were calculated to be 6.9 Kcal/mole/deg. and 8.0 Kcal/mole/deg. respectively. Km of sucrose and inulin hydrolyses was 6.7mM and 8.0mM. The ratio of Vmax of sucrose hydrolysis to that of inulin hydrolysis was 2.3. The mode of hydrolytic action on inulin by inulase showed to be single chain mechanism. By enzymatic hydrolysis of Jerusalem Artichoke ($\mbox{\underline{Helithus}}$ $\mbox{\underline{tuberosus}}$ $\mbox{\underline{L}}$.) tuber extract which contained inulin type fructose polymers, high fructose sugar mixture which contained 28.8g of fructose and 8.7g of glucose was obtained. The separation of fructose was carried out by Dowex 1-X8 ion-exchange chromatography. After separation 24.5g of pure fructose was obtained from 100g of dried artichoke with the overall recovery of 61\% on the basis of fructose in dried tuber.
돼지감자내에 존재하는 inulin으로 부터 과당을 생성하기 위하여 가수분해 효소인 inulase 를 Saccharomyces fragilis에서 제조하였다. 돼지 감자 추출액 배지에서 효모를 배양한후 배지내에 분비된 inulase 를 원심분리, 냉동건조하여 조효소를 만들고 다시 Sepharose 6B 로 겔여과한 후, DEAE-Sephadex A50 을 사용하여 효소를 정제하였다. 전기영동상으로 정제한 효소는 순수함을 알수 있었고 또한 탄수화물을 구성물질로 하는 당 단백질임이 밝혀졌다. 이효소는 기질에따라 pH와, 온도영향이 달랐으나 inulin 과 sucrose 를 다 분해함을 알수있었다. inulin 가수분해의 양성은 single-chain mechanism 임을 확인하였다. 돼지감자내 존재하는 inulin 의 분해는 표준 inulin보다 훨씬 빨랐으나 생성물중 과당의 비율은 다소 떨어졌다. 효소에 의한 가수분해결과 생성된 포도당과 과당의 혼합물로 부터 Dowex 1-x8이온 교환수지에 의해 순수한 과당을 얻었다. 그 결과 100g의 건조돼지감자로 부터 24.5 g의 과당을 얻어 과당을 기준으로 할 때 61%의 수율을 얻었다.
